5%地克珠利纳米乳对鸡球虫病的疗效研究

Research of efficacy of 5% diclazuril nanoemulsion against chicken coccidiosis

【目的】研究5%地克珠利纳米乳对鸡球虫病的疗效。【方法】选择300只18日龄的雏鸡,随机均分为5%地克珠利纳米乳高、中、低剂量组,0.5%地克珠利溶液组,阴性对照组和阳性对照组6组,试验组和阳性对照组每只鸡经口接种柔嫩艾美耳球虫(Eimeria tenella)卵囊悬液0.5mL(含球虫卵囊1.0×105个),阴性对照组接种等量生理盐水,感染48h后饮水给药,按地克珠利计,5%地克珠利纳米乳高、中、低剂量组给药质量浓度分别为2.0,1.0,0.5mg/L,0.5%地克珠利溶液组给药质量浓度为1.0mg/L,给药5d,阴性对照组和阳性对照组正常饮水。人工感染后第8天测定相对增重率、存活率、盲肠病变值、卵囊值和抗球虫指数。【结果】5%地克珠利纳米乳中剂量组疗效最好,抗球虫指数达195.81,相对增重率达95.81%,存活率为100%,病变值为0,卵囊值为0。【结论】5%地克珠利纳米乳是一种高效抗球虫药,临床推荐用量1.0mg/L(按地克珠利计),饮水给药。

【Objective】 The research was to study the efficacy of 5% diclazuril nanoemulsion against chicken coccidiosis.【Method】 Three hundred 18-day-old chickens were randomly divided into high,middle,low doses of 5% diclazuril nanoemulsion group,0.5% diclazuril solution group,negative control group and positive control group.Each chicken of the experimental group and positive control group was inoculated 0.5 mL Eimeria coccidium oocyst suspension（including 1.0×105 oocysts） and the negative control group was given equivalent physiological saline.After 48 h,high,middle and low doses of 5% diclazuril nanoemulsion group was separately given 2.0,1.0,0.5 mg/L diclazuril by drinking water and 0.5% diclazuril solution group was given 1.0 mg/L diclazuril by drinking water.The drug administration lasted for 5 d.The negative and positive control group were given drinking water normally.Relative weight gain rate,livability,cecum lesion value,oocyst value and ACI（anticoccidial index） were determined on the eighth day after artificial infection.【Result】 Efficacy of middle dose of 5% diclazuril nanoemulsion was the best,ACI being 195.81,relative weight gain rate 95.81%,livability 100%,cecum lesion value 0 and oocyst value 0.【Conclusion】 5% diclazuril nanoemulsion is an efficient anticoccidial drug.