AChE及M_1受体在柄袋沙蠋胚胎和幼虫中的免疫组化定位

Immunohistochemical Localization of AChE and M_1 Receptor in the Embryos and Larvae of Arenicola brasiliensis

采用免疫组织化学S-ABC法和兔抗鼠抗体研究了乙酰胆碱酯酶(AChE)及M1受体在柄袋沙蠋(Arenicola brasiliensis)胚胎和幼虫的分布。结果表明,AChE及M1受体在柄袋沙蠋胚胎和幼虫中分布非常广泛,从未受精卵开始,直至研究的5刚毛节幼虫均有分布,但不同发育阶段分布的情况不同。卵裂期主要分布于细胞膜和核的周围,幼虫期主要分布于头区、纤毛着生处、刚毛着生处和消化道等部位。另外,实验结果显示,M1受体的分布与AChE的分布呈现出对应一致的特点。AChE及M1受体在柄袋沙蠋胚胎和幼虫中分布的广泛性,表明乙酰胆碱可能参与柄袋沙蠋生长发育的调节。

By using immunohistochemical S-ABC technique,AChE and M1 receptor in the embryos and larvae of Arenicola brasiliensis were detected.The results showed that AChE and M1 receptor were positivity stained from unfertilized eggs to 5-setiger larvae.However,their distribution was distinctive at different phases.For example,at the cleavage stage AChE and M1 receptors were mainly distributed in the plasma membrane and perinuclear cytoplasm,while at the larval stage they were mainly detected in the head region,digestive tract,the areas where cilia grew,and setae etc.Furthermore,AChE and M1 receptors had a similar distribution pattern in embryos and larvae.The results indicate that ACh may act as an important regulator during embryo and larvae development.