低强度复合振动治疗去卵巢老龄大鼠骨质疏松实验研究

Low-magnitude Compound Vibration Treated Ovariectomized Old SD Rats Osteoporosis:An Experimental Study

目的:观察低强度复合振动对去卵巢老龄大鼠骨质疏松的治疗作用。方法:13月龄SD雌性经产大鼠16只,随机均分为对照组和实验组。卵巢摘除法骨质疏松造模,3个月后检查骨密度(BMD)下降后实验组接受低强度复合振动(振动频率45 Hz～55 Hz,振动强度0.12 g～0.21 g,g=9.8 m/s2,20分钟/次/天,5天/周),对照组不进行振动治疗。13周复合振动实验结束后分别活体测量两组大鼠腰椎、股骨骨密度(Lorland XR-46),胫骨标本骨微结构扫描分析(SCANCO u-CT),测试腰椎、股骨标本力学性能(858 mini bionix)及酶法检测血清碱性磷酸酶、非前列腺酸性磷酸酶,电化学发光免疫法检测血清骨钙素和I型胶原C端交联肽。结果:复合振动实验结束后实验组腰椎和股骨BMD较实验前显著增加(分别增加0.0110±0.0048 g/cm2,P=0.000和0.0056±0.0065 g/cm2,P=0.004),而对照组显著下降(分别下降0.0071±0.0042 g/cm2和0.0073±0.0063 g/cm2)。相较对照组,骨微结构参数Tb.N显著增加(P=0.002)、Tb.Sp显著降低(P=0.005)以及BV/TV显著增加(P=0.048),腰椎、股骨强度极限也显著增加(P=0.038,P=0.014),但血清骨钙素、碱性磷酸酶、I型胶原C端交联肽、非前列腺酸性磷酸酶两组无统计学差异。结论:低强度复合振动可以减轻去卵巢老龄大鼠骨质疏松程度,提高骨质疏松大鼠骨质量。

Objective To observe the effect of low-magnitude compound vibration to ovariectomized old SD rat＇ s osteoporosis.Methods Sixteen 13-months-old multiparous SD rats were randomly divided into 2 groups： control（n = 8） and vibration group（n = 8）.All the rats were ovariectomized to model osteoporosis for 3 months.The experiment of low-magnitude compound vibration began when bone mineral density（BMD）decreased.The vibration group received low-magnitude compound vibration（vibration frequency：45 Hz～55 Hz,vibration magnitude：0.12 g ～ 0.21 g,g = 9.8 m/s2,20 mins/times·day,5 days/week） and the control group kept sedentary.The parameters related to osteoporosis of the two groups rats were measured after the 13 weeks compound vibration trial,such as：the intravital lumbar and femur BMD（Lorland XR-46）,bone microarchitecture of tibia specimen（SCANCO u-CT）,biomechanical property of lumbar vertebra and femur（858mini bionix）,blood serum alkali phosphatase and acid phosphatese of non-prostate（enzymic method）,blood serum osteocalcin and carboxyterminal crosslinked telopeptide of typeⅠcollagen（electrochemiluminescence immunization）.Results Lumbar and femur BMD of vibration group rats increased significantly（0.0110±0.0048 g/cm2,P =0.000 and 0.0056±0.0065 g/cm2,P = 0.004,respectively） at post-vibration compared with pre-vibration,while the control group decreased accordingly（-0.0071±0.0042 g/cm2 and-0.0073±0.0063 g/cm2）.Significant improvement were found on some parameters of bone microstructure and bone biomechanics,such as Tb.N（P = 0.002）,Tb.Sp（P = 0.005）,BV/TV（P = 0.048） and bone ultimate strength（P = 0.038,0.014）.But there was no statistics difference on the bone metabolism markers between the two groups.Conclusion Low-magnitude compound vibration could slow the process of osteoporosis and enhance the bone quality of ovariectomized old rats.