捻转补泻手法对应激性高血压大鼠CGRP、NO含量的影响

Effects of reinforcing and reducing methods by twirling and rotating the needle on contents of CGRP and NO in rats with stress-induced hypertension

目的:探讨捻转补泻手法对应激性高血压大鼠的效应差异及其作用机制。方法:雄性Wistar大鼠60只,随机分为5组,每组12只,分别为正常组(A)、模型对照组(B)、针刺对照组(C)、针刺补法组(D)、针刺泻法组(E)。应激性高血压大鼠造模成功后,C组针刺左侧"太冲"穴,不行针,不做手法;D组针刺左侧"太冲"穴并用捻转补法;E组针刺左侧"太冲"穴并用捻转泻法。A、B两组大鼠不做针刺处理。7日后观察各组大鼠血浆及下丘脑降钙素基因相关肽(CGRP)、一氧化氮(NO)含量的变化。结果:针刺处理后,对大鼠血浆、下丘脑CGRP含量的影响:B组血浆CGRP含量为(261.0±20.1)pg/mL、下丘脑CGRP含量为(156.0±21.8)pg/mg,C组分别为(255.3±33.6)pg/mL、(154.3±47.3)pg/mg,两组与A组的(375.9±36.5)pg/mL、(213.6±50.1)pg/mg比较,CGRP含量下降(均P<0.05);E组的(465.9±53.2)pg/mL、(250.74±47.7)pg/mg与C组及D组的(283.3±29.8)pg/mL、(164.6±47.4)pg/mg比较,CGRP含量明显升高(均P<0.05)。C组与B组、D组比较,CGRP含量无明显变化(均P>0.05)。对大鼠血浆、下丘脑NO含量的影响:B组分别为(43.7±5.5)μmol/L、(23.3±2.9)μmol/g,C组为(46.5±6.4)μmol/L、(23.1±3.3)μmol/g,D组为(45.8±6.7)μmol/L、(25.0±2.7)μmol/g,3组分别与A组的(64.5±9.9)μmol/L、(30.7±1.6)μmol/g比较,NO含量下降(均P<0.05);E组的(55.9±4.6)μmol/L、(30.4±2.1)μmol/g与C组及D组比较,NO含量升高(均P<0.05)。C组与B组、D组比较,NO含量变化差异无统计学意义(均P>0.05)。结论:针刺泻法可以有效地升高应激性高血压大鼠CGRP、NO含量,从而降低其血压,针刺补法对应激性高血压大鼠降压作用不明显。捻转补法与泻法具有不同的效应。

Objective To explore the different effects of reinforcing and reducing methods by twirling and rotating the needle on stress-induced hypertension and its mechanism. Methods Sixty male Wistar rats were randomly divided into a nomal group （group A）, a model group （group B）, an acupuncture control group （group C）, an acupuncture with reinforcing method group （group D）and an acupuncture with reducing method group （group E）, 12 rats in each group. The model of stress-induced hypertension was established by foot-shock and noise stimulation in the other groups except group A. Group C was treated by acupuncture at left ＂Taichong＂ （LR 3） with no manipulation, group D with twirling reinforcement method and group E with twirling reducing method at the same aeupoint, respectively, and group A and B with no acupuncture treatment. The contents of calcitonin gene-related peptide （CGRP） and nitrogen monoxidum （NO） in blood plasma and hypothalamus after 7 days were observed. Results After acupuncture treatment, thecontents of CGRP in blood plasma and hypothalamus were （375.9± 36.5） pg/mL and （213.6± 50. 1） pg/mg in group A, which were higher than （261.0 ± 20. 1） pg/mL and （156.0±21.8） pg/mg in group B, and （255. 3±33.6） pg/mL and （154.3±47. 3） pg/mg in group C （ all P 0.05）, and （465.9±53, 2） pg/mL and （250.74±47.7） pg/mg in group E higher than those in group C and （283.3±29, 8） pg mL and （164.6±47.4） pg/mg in group D （all P〈0.05）. and there was no significant difference among group B, C and D （all P〈0.05）. After acupuncture treatment, the contents of NO in blood plasma and hypothalamus were （43.7±5.5） tzmol/L and （23.3±2.9） μmol/g in group B, （46. 5±6.4） μmol/L and （23. 1±3.3） μmol/g in group C, （45.8±6. 7） μmol/L and （25.0±2. 7）μmol/g in group D, which were all lower than （64.5±9.9） μmol/L and （30.7±1.6）μmol/g in group A （all P〈0. 05） （55.9±4.6） μmot/L and （30.4±2.1） μmol/g in group E higher than those in group C and group D （all P〈0.05）, and there was no significant difference among group B, C and D either （all P〉0.05）. Conclusion Acupuncture with twirling reducing method can increase the contents of CGRP and NO in rats with stress-induced hypertension, thereby causing a fall in blood pressure, however, no significant influence with twirling reinforcing method. Reinforcing or reducing method by twirling and rotating the needle has different efficacy.