摘要
通过ＳＤＳＰＡＧＥ、免疫印迹和相加ＥＬＩＳＡ分析了五株抗伪狂犬病病毒杂交瘤细胞系所分泌的单抗５Ｈ２、２Ｅ６、２Ｇ１１、３Ｄ１０和１Ｂ５（均为ＩｇＧ１）各自所识别的病毒多肽。结果证明：单抗５Ｈ２、２Ｅ６、２Ｇ１１和３Ｄ１０识别伪狂犬病病毒９７ＫＤ多肽，单抗１Ｂ５识别５４ＫＤ多肽。相加ＥＬＩＳＡ证实单抗５Ｈ２、２Ｅ６、２Ｇ１１和３Ｄ１０识别同一多肽的重叠表位或邻近表位。
The polypeptides of Pseudorabies virus(PrV) were analyzed by SDS PAGE,Western blotting and an additive ELISA,using 5 anti PrV monoclonal antibodies (McAbs) Secreted by the hybridoma cell lines 5H 2、2E 6、2G 11 、3D 10 、1B 5,which fell into the subclass of IgG 1.The results showed that McAbs 5H 2、2E 6、2G 11 and 3D 10 can recognize 97KD polypeptide of PrV,and McAb 1B 5 can recognize 54KD polypeptide. The additive ELISA indicated that McAbs 5H 2、2E 6、2G 11 and 3D 10 could recognize the overlapping or closely adjacent epitodes of the same polypeptide.
出处
《青海畜牧兽医杂志》
1999年第4期20-22,共3页
Chinese Qinghai Journal of Animal and Veterinary Sciences
关键词
伪狂犬病病毒
单克隆抗体
特性
Pseudorabies Virus,Monoclonal Antibodies,Property
