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雷帕霉素联合顺铂对CCL19调节头颈鳞状细胞癌细胞活性的影响

Effect of rapamycin combined with cisplatin on head and neck squamous cancer cells regulated by CCL19
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摘要 目的 探讨雷帕霉素(rapamycin)与顺铂(cisplatin)联合使用对趋化因子CCL19调节头颈鳞状细胞癌细胞活性的影响.方法 应用甲基噻唑基四唑(MTT)法及流式细胞仪检测顺铂及雷帕霉素对CCL19诱导后人头颈鳞状细胞癌淋巴结转移细胞系(PCI-4B、PCI-37B)生长抑制作用、细胞凋亡作用和细胞周期变化.使用Calcusyn软件计算两种药物(浓度比为80:1)联合使用时的剂量效应参数及联合指数(combination index,CI),使用SPSS 11.0软件包进行统计分析.结果 顺铂及雷帕霉素可以分别提高CCL19作用后细胞的生长抑制率(CCL19+顺铂作用后两细胞系的生长抑制率为21.22%±2.68%和22.76%±2.79%,CCL19+雷帕霉素作用后为19.46%±2.54%和20.6%±4.38%)、细胞G1期比例(CCL19+顺铂作用后两细胞系的G1期比例为72.14%±2.66%和76.39%±1.45%,CCL19+雷帕霉素作用后为75.26%±5.92%和74.00%±1.51%)和凋亡率(CCL19+顺铂作用后两细胞系的凋亡率9.59%±0.86%和7.55%±0.66%,CCL19+雷帕霉素作用后为8.21±1.80%和6.26±0.78%).两种药物浓度比为80:1联合使用时,PCI-4B细胞在半数抑制量(inhibitory concentration 50%,IC50)以下,CI<1,IC75以上CI>1;PCI-37B则在IC75以下CI<1,IC90以上CI>1.结论 顺铂及雷帕霉素均有抑制CCL19对人头颈鳞状细胞癌淋巴结转移细胞的活性调节作用,并且二者联合使用存在协同作用. Objective To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine( C-C motif) ligand 19 (CCL19). Methods The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry(FCM). Dose-effect relationship parameters and combination index(CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.Results Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G1 phase and apoptosis of CCL19 induced cancer cells (P <0. 05 ). Under inhibitory concentration 50% (IC50), CI was less than 1, and in IC75, it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC75, CI was less than 1, and in IC90, it was more than 1. Conclusions Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2011年第4期197-200,共4页 Chinese Journal of Stomatology
基金 国家自然科学基金(30672331) 辽宁省教育厅高等学校科研项目计划(2009A755)
关键词 趋化因子 CC 鳞状细胞 顺铂 西罗莫司 Chemokines,CC Carcimona,squamous cell Cisplatin Siromus
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  • 1Greenlee RT,Hill-Harmon MB,Murray T,et al.Cancer statistics,2001.CA Cancer J Clin,2001,51 (1):15-36.
  • 2Liu FY,Zhao ZJ,Li P,et al.Mammalian target of rapamycin (mTOR) is involved in the survival of cells mediated by chemokine receptor 7 through PI3K/Akt in metastatic squamous cell carcinoma of the head and neck.Br J Oral Maxillofac Surg,2010,48(4):291-296.
  • 3赵震锦,刘法昱,孙长伏.趋化因子受体7小干扰RNA对人头颈部鳞状细胞癌细胞增殖及侵袭的影响[J].中华口腔医学杂志,2009,44(1):5-10. 被引量:3
  • 4刘法昱,赵震锦,黄绍辉,孙长伏.NF-κB抑制剂PDTC对CCL19调控人头颈鳞癌细胞增殖活性的作用[J].中国医科大学学报,2008,37(6):847-849. 被引量:4
  • 5刘法昱,赵震锦,李鹏,丁雪,孙长伏.趋化因子CCL19对体外培养人头颈鳞癌细胞活性的影响[J].上海口腔医学,2010,19(2):158-161. 被引量:5
  • 6李尧,戚忠政,尚德浩,孙长伏.NS398对SACC-83细胞抑制作用实验研究[J].中国实用口腔科杂志,2010,3(2):85-87. 被引量:2
  • 7Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors.Adv Enzyme Regul,1984,22:27-55.
  • 8Vézina C,Kudelski A,Sehgal SN.Rapamycin (AY-22,989),a new antifungal antibiotic.I.Taxonomy of the producing streptomycete and isolation of the active principle.J Antibiot (Tokyo),1975,28(10):721-726.
  • 9Sehgal SN.Rapamune (Sirolimus,rapamycin):an overview and mechanism of action.Ther Drug Monit,1995,17(6):660-665.
  • 10Herberger B,Berger W,Puhalla H,et al.Simultaneous blockade of the epidermal growth factor receptor/mammalian target of rapamycin pathway by epidermal growth factor receptor inhibitors and rapamycin results in reduced cell growth and survival in biliary tract cancer cells.Mol Cancer Ther,2009,8 (6):1547-1556.

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