长春市某小学7～12岁儿童福赛斯坦氏菌和中间普氏菌分布状态调查

Colonization of Tannerella forsythus and Prevotella intermedia in dental plaque samples from children of Changchun Ziqiang primary school

目的 应用聚合酶链反应（PCR）法对儿童口腔内福赛斯坦氏菌（Tannerella forsythus,Tf）和中间普氏菌（Prevotella intermedia,Pi）进行检测,探讨检出结果与牙周临床指标之间的关系.方法 选取长春市自强小学151名7至12岁儿童为研究对象,选择右上颌中切牙和第一磨牙为被检牙位,取龈上菌斑、记录探诊出血（bleeding on probing,BOP）、探诊深度（probing depth,PD）、牙龈指数（gingival index,GI）,应用PCR法对儿童龈上菌斑中的Tf和Pi进行检测.结果 儿童龈上菌斑中Tf、Pi的检出率分别为40.3%（1 18/293）、46.4%（136/293）;6颊面Tf、Pi的检出率分别为55.2%（80/145）、53.8%（78/145）均显著高于（1）唇面的Tf和Pi的检出率[分别为25.7%（38/148）、39.2%（58/148）],差异有统计学意义（P＜0.01）;Pi检出率随年龄增长呈逐渐减低趋势,Tf检出率在7～8岁组最高,其次为11～12岁和9～10岁组;BOP阳性部位的Tf、Pi检出率[分别为43%（35/81）、49%（40/81）]均高于BOP阴性部位二者的检出率[分别为37%（72/194）、45%（87/194）].在BOP阳性部位,随PD加深Tf检出率逐渐增高,特别是在PD 4 mm以上时Tf检出率明显增高（P＜0.01）,Tf检出率与BOP阳性、PD增加呈正相关.结论 7～12岁儿童龈上菌斑中高频度分布着Tf和Pi;上颌前牙区与磨牙区菌丛构成不同,Tf、Pi在磨牙区检出率更高,Tf的检出率与PD呈正相关,Tf检出率在7～8岁组最高,Pi检出率随年龄增长呈减低趋势,儿童时期进行牙周病早期预防是非常必要的.

Objective To detect the presence of Tannerella forsythus（Tf）and Prevotella intermedia （Pi） using polymerase chain reaction（PCR） in the oral plaque samples from children and investigate the relationship between bacteria and clinical parameters. Methods A total of 151 children aged 7 to 12 years were selected from Changchun primary school. The supragingival plaque sample was collected from the mesiobuccal and labial surfaces of the right maxillary central incisor （ FDI 1 ） and the right maxillary first molar （ FDI 6 ）. Extracted DNA from plaque samples was used for PCR analysis. Intraoral examination,probing depth （PD） and bleeding on probing （BOP） were performed and recorded. Results The detection rate for Tf was 40. 3% （ 118/293 ） and Pi was 46. 4% （ 136/293 ） in supragingival plaque. The detection rates for Tf and Pi in molars were much higher than those in incisors（ P ＜0. 01 ）. The detection rate of Tf and Pi was positively related to BOP ＋ and PD. The detection rate for Pi decreased gradually with age, and the detection rate for Tf was highest in the group aged 7 to 8 and the detection rates for Tf and Pi were higher in the gingiva with BOP ＋ than that with BOP - （ P ＞ 0. 05 ）. The detection rates for Tf increased remarkably with BOP ＋ and especially when PD was greater than 4 mm. Conclusions Detection rates of putative periodontal pathogens from healthy children of 7 to 12 years of age were high. The detection rates for Tf and Pi in molars were much higher than those in incisors, and the presence of Tf and Pi in supragingival plaque was related to periodontal parameters.