自体基质血管成分改善脂肪组织移植效果的实验研究

Experimental study on stromal vascular fraction(SVF) cells to improve the effect of autologous fat tissue transplantation

目的:分析自体脂肪基质血管成分(stromal vascular fraction cells,SVF)对脂肪颗粒(adipose granule,AG)移植的作用。方法:从6只健康新西兰家兔背部肩胛区获取脂肪组织,实验组将自体SVF与自体AG复合,植入家兔耳部皮下,对照为单纯脂肪移植。在术后1、3、6个月,用B超和游标卡尺测量移植脂肪体积;术后6个月取材常规组织学观察。结果:术后1、3、6个月对照组脂肪组织存活率分别为:(64.35±8.36)%、(58.22±2.88)%、(50.61±9.47)%;实验组脂肪组织存活率分别为:(77.42±5.1)%、(67.95±6.09)%、(72.75±4.37)%。两组比较均存在显著性差异(P<0.05)。术后6个月组织学观察两组呈正常脂肪组织形态,未见明显差异。结论:自体SVF复合脂肪颗粒能够显著提高移植脂肪组织的成活率,为临床脂肪移植提供实验依据。

Objective The present study evaluated the role of stromal vascular fraction（SVF） cells on autologous adipose granule（AG） transplantation.Methods Adipose tissue was harvested in the scapular region of 6 healthy New Zealand white rabbits.Stromal vascular fraction（SVF） cells combined with autologous adipose granule were implanted in the ears of the rabbits as the experiment group.Pure adipose granule was as the control group.The volume of adipose tissue was evaluated 1,3 and 6 months after surgery by B-mode ultrasound and caliper.Both ears of the rabbits were compared histologically 6 months after surgery.Results 1、3、6 months after surgery,the survival rates of the control group were（64.35±8.36）%、（58.22±2.88）%、（50.61±9.47）%;the survival rates of the experiment group were（77.42±5.1）%、（67.95±6.09）%、（72.75±4.37）%.The differences of the two groups were statistical significant（P0.05）.6 months after surgery,HE staining of both groups was similar to normal adipose tissue and there was no significant difference between the two groups.Conclusion Autologous stromal vascular fraction（SVF） cells can improve the survival rate of transplanted fat tissue and provide experimental basis for clinical fat transplantation.