微流控免疫芯片检测方法的研究进展

Developments in Microfluidics-based Immunoassays

微流控免疫芯片以其微型化、高通量、快速检测及低消耗等优点成为近年来分析领域的研究热点.检测技术是微流控芯片的重要组成部分之一.本文重点综述了近年来微流控免疫芯片的微系统研究及相应的检测方法和技术,包括电化学检测及荧光检测、紫外-可见吸收光谱检测、化学发光和生物发光检测、表面增强拉曼散射检测、光纤检测、表面等离子体共振谱检测、热透镜显微镜检测和比色检测等光学检测及其它新型检测方面的进展,并展望了其发展前景.

Microfluidics-based immunoassays have become an increasing research field in recent years owing to their advantages of miniaturization,high throughput,fast detection and low consumption.Detection is an important part in microfluidics-based immunoassays.Recent advances in the studies of micro-systems and detection methods,including electrochemical detection,fluorescence spectrometry,ultraviolet-visible spectrometry,chemiluminescence and bioluminescence,surface-enhanced Raman scattering,fiber-optic sensor,surface plasmon resonance,thermal lens microscopy,colorimetry and other new detection methods applied in microfluidics-based immunoassays,were summarized and reviewed in this paper.The prospects of the detection methods for microfluidics-based immunoassays were presented.