罗丹明B与基因共负载纳米粒子的制备与性能

Construction of Rhodamine B and Gene Co-delivery Nanoparticles

以疏水性荧光染料罗丹明B为模型药物,采用聚乙烯亚胺-接枝-胆固醇(PEI-g-Chol)两亲聚合物作为药物载体,成功制备了罗丹明B与基因共负载的超分子组装体.通过组装条件的调控,获得了具有良好DNA缔合特性的尺寸为154 nm,表面电位为33.7 mV的球形纳米粒子.细胞培养实验结果表明,表面带正电荷的罗丹明B与基因共负载纳米粒子很容易被细胞内吞,并能有效转染细胞,为高效安全的药物与基因共负载微载体的制备提供了切实可行的途径.

The hydrophobic fluorescent dye rhodamine B was chosen as a model drug.PEI-g-cholesterol amphiphilic polymer was synthesized as drug carrier.By selecting proper conditions,rhodamine B and gene co-delivery nanoparticles were successfully constructed via supramolecular assembly.The co-delivery particles with diameter about 154 nm and ξ-potential about 33.7 mV could effectively condense DNA.The in vitro cell culture experiments indicated that the positive co-delivery nanoparticles were easily uptaked by HEK293 cells and showed effective transfection efficiency.