荧光定量PCR检测巨细胞病毒感染的标本选择

Selection of cytomegalovirus infection samples detected by quantitative PCR

目的优化检测标本,提高巨细胞病毒的阳性检出率,为临床诊断和治疗提供可靠依据。方法实时荧光定量PCR检测患者血、淋巴细胞和尿中的人巨细胞病毒DNA(HCMV-DNA),比较其阳性检出率。结果通过实时荧光定量PCR检测的572例患者标本中血浆标本300例,HCMV-DNA阳性45例,阳性率为15.0%;淋巴细胞标本176例,HCMV-DNA阳性30例,阳性率为17.0%;尿标本96例,HCMV-DNA阳性60例,阳性率为62.5%;45例患者配对检测血、尿标本HCMV-DNA,血HCMV-DNA阳性7例,阳性率15.6%,尿HCMV-DNA阳性率64.4%,采用χ2检验显示,血浆、淋巴细胞HCMV-DNA的检出率差异无统计学意义,而血和尿标本HCMV-DNA的检出率差异有统计学意义(P<0.05)。结论采用实时荧光定量PCR检测患者巨细胞病毒感染,以尿作为检测材料的阳性率高于淋巴细胞及血浆,可提高HCMV-DNA的阳性检出率。

OBJECTIVE To optimize the test samples,to improve the detection of CMV-positive rate and provide a reliable basis for clinical diagnosis and treatment.METHODS Blood,lymphocytes and urine human cytomegalovirus DNA（HCMV-DNA） were detected with real-time fluorescence quantitative PCR,and the positive rates were compared.RESULTS A total of 572 patients samples,blood samples of 476 cases,45 cases of plasma-positive,the positive rate was 15.0%;lymphocytes detected in 30 cases,the positive was 17.0%;urine samples of 96 cases,60 cases positive,the positive was 62.5%;of which 45 cases were also collected blood and urine samples were detected by fluorescence quantitative PCR,blood-positive and 7,the positive was 15.6%,urine-positive,the positive was 64.4%.X2 test showed the difference of lymphocytes detection rate of HCMV had no statistivally significance（P0.05）,while the detection rates of blood and urine HCMV samples were significantly significant（P0.05）.CONCLUSION Adopting real-time fluorescence quantitative PCR detection of cytomegalovirus infection and using urine testing materials as the positive rate in lymphocyte and plasma can increase the positive detection rate of HCMV.