茶叶特征成分对小鼠酒精化学损伤修复作用的动物实验研究初报

Preliminary Study on the Repairing Effects of Characteristic Constituents of Tea Against Chemical Injury of Alcohol in Mice

探讨以茶氨酸、γ-氨基丁酸(GABA)等茶叶特征成分为主要配方的复合氨基酸颗粒剂(以下简称功能修饰因子)对急性酒精中毒小鼠醉酒时间、醉酒率、血液生物化学指标以及肝组织、胃组织代谢酶的影响,为进一步开展功能修饰因子的功效性研究奠定基础。实验结果表明:排除小鼠实验中应激反应等不利因素,与模型组比较,不同灌胃剂量功能修饰因子(0.15ml/10g、0.18ml/10g)不同灌胃浓度(5.35mg/mL、10.7mg/mL、21.4mg/mL)均能明显降低小鼠醉酒率,延长醉酒时间(P<0.05或P<0.01)与对照组比较,功能修饰因子各剂量组可显著降低血清ALT、AST水平,减少胃、肝组织MDA生成、增加SOD的活性,显著升高大量饮酒后小鼠肝组织ADH活性,促进酒精代谢,降低血酒浓度显示功能修饰因子在减缓及降低酒精化学损伤方面有突出功效。

To observed the effect of the formula compounded with amino acid granules of L-theanine,γ- aminobutyric（GABA）and other characteristic constituents of tea（hereinafter referred to as functional modification factor）on ebriety rate,biochemical index of blood,liver tissue,metabolism enzyme in stomach tissue of mice in the state of acute alcoholisms were studied.The results showed that：Feeding with different garage doses of different function modifier（0.15ml/10g,0.18ml/10g）containing respective concentrations （5.35mg/mL, 10.7mg/mL, 21.4mg /mL）could decrease the ebriety rate in mice obviously and the mice could endure a more long time under the action of alcohol（P〈0.05 or P〈0.01）investigated by means of eliminating the stress response in mice and other unfavorable factors and being compared with model group,Compared with the control group,function of the dose modification factors could significantly reduce serum ALT,and AST levels,as well as MDA production in stomach and liver tissue,bul increase SOD activity significantly.When the mice drinked a lot of activity,The liver ADH,were reduced the blood alcohol concentration.This demonstrate that modifying factors possess mitigation and reduction of alcohol and had a distinct effect of protection for liver.