黑龙江省种植品种中稻瘟病抗性基因Pib和Pita的分布

Distribution of two blast resistance genes Pib and Pita in major rice cultivars in Heilongjiang Province in China

利用源于Pib和Pita基因本身的特异性分子标记,检测了2009年黑龙江省种植面积0.67万hm2以上的36份水稻品种的Pita和Pib抗性基因型。结果表明,垦稻13、垦稻14、龙粳22、松粳9等4份材料含有Pib基因,而不含有Pita;东农428、龙稻5、龙粳18、龙粳28、绥粳4、绥粳9等6份材料含有Pita基因,而不含有Pib基因。同时用黑龙江省稻瘟病优势混合菌接种鉴定,结果表明Pib、Pita在黑龙江有较高利用价值。该结论有助于含Pib、Pita基因的品种合理布局及利用,为抗病基因聚合育种打下了基础。

The two major blast resistance genes,Pib and Pita,showed good resistance spectrum to rice blast in many rice growing areas of China,and have been utilized in rice breeding and production.However,the distribution of these two genes in rice of Heilongjiang Province had not been well investigated,resulting in the blindness of their utilization in rice breeding.In this study,the existen-ces of the two genes in 36 rice cultivars/lines were rapidly determined using the dominant DNA marker of Pita/pita and Pib/pib alleles.The Pib gene was found in four cultivars.i.e.Kendao13,Kendao14,Longjing22,Songjing9.The Pita gene was found in other six cultivars i.e.Dongnong428,Longdao5,Longjing18,Longjing28,Suijing4 and Suijing9.These results were useful for rational utilization and layout of the rice cultivars with the Pita and Pib genes in cold region,and for incorporating and stacking these genes into elite present leading cultivars/lines by marker assisted selection in rice breeding.