摘要
利用54对欧榛SSR引物,对榛属12个种、平欧杂交榛及平平欧杂交榛共64份榛属资源进行了遗传多样性及亲缘关系分析。结果表明,欧榛遗传变异丰富,64份榛属资源18对SSR引物在欧榛10个样本中共检测到95个等位基因,每个位点的等位基因数2～10个,观测杂合度值(Ho)和期望杂合度值(He)的变化范围分别为0.214～0.816和0.348～0.800。聚类分析结果显示,64份榛属种质可划分为6组,平榛与欧榛亲缘关系最近。由此可知,欧榛SSR标记是榛属植物资源遗传分析的有力工具,为平榛资源的遗传改良在分子水平上提供了理论依据和基础。
Genetic diversity and cluster analysis of 64 hazelnut germplasm materials were studied with SSR molecular markers for Corylus avellana in this study.The results showed that genetic variation of Corylus avellana germplasm was abundant,95 alleles were detected in 10 Corylus avellana materials,the number of alleles each loci changed from 2 to 10,the range of Ho and He was 0.214-0.816 and 0.348-0.800,respectively.The results of cluster analysis showed 64 hazelnut materials were divided into six groups,the genetic relationship between Corylus heterophylla and Corylus avellana was close.The study proved that SSR molecular markers for Corylus avellana were useful tools for genetic analysis of hazelnut germplasm,and this would provide theoretical basis and useful analysis tools for Corylus heterophylla germplasm genetic improvement.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第4期129-136,共8页
Journal of Northeast Agricultural University
基金
黑龙江省自然科学基金(C200926)
佳木斯大学科技开发项目(KF2010-039)
关键词
欧榛
SSR标记
亲缘关系分析
遗传改良
Corylus avellana
SSR molecular marker
phylogenetic analysis
genetic improvement
