摘要
目的:建立溃疡性结肠炎兔模型并探讨其应用价值。方法:30只成年健康新西兰兔随机分为5组,每组6只,实验组分别用5%乙酸、8%乙酸、10%乙酸灌肠造模,阳性对照组以三硝基苯磺酸(TN-BS)灌肠造模,空白对照组以生理盐水灌肠,比较各组疾病活动指数(DAI),并进行组织学观察。结果:5%乙酸造模组DAI与TNBS对照组无统计学差异(P>0.05),10%乙酸造模组与其他各组差异均有统计学意义(P<0.05~0.01)。组织学观察:除空白对照组外,各组均发现肠壁增厚,黏膜弥漫性出血、溃疡、坏死等情况,其中5%乙酸造膜组黏膜炎症细胞浸润,腺体消失,而另外3个模型组黏膜均严重坏死脱落。结论:5%乙酸局部刺激能较好复制兔溃疡性结肠炎模型,该模型制作方法简单,成本低,周期短,成功率高,重复性好,是较为理想的溃疡性结肠炎动物模型。
Objective:To establish a rabbit model of ulcerative colitis.Methods:Thirty adult white rabbits of New Zealand were divided into five groups randomly,each having 6 animals.The experimental groups were treated with 5%,8% and 10% acetic acid to induce the enema modeling.TNBS enema modeling(positive control group) and negative control group were also created for comparison of DAI among these groups.Historical analysis was also performed.Results:The group treated with 5% acetic acid showed no significant difference in DAI comparing with the positive control group(P0.05),but the group treated with 10% acetic acid group showed significant differences when comparing with the rest 4 groups(P0.05~0.01).Histological observation revealed that increased intestinal walls,mucosal bleeding,ulcers,and necrosis.Specifically,the 5% acetic acid treated group showed inflammatory,cell infiltration and disappearance of glands,etc.The other 3 groups also showed mucosal necrosis and fall.Conclusions:Topical stimulation of 5% acetic acid can induce ulcer colitis in rabbit model.It's also cheap,quick and has good reproducibility.
出处
《海南医学院学报》
CAS
2011年第3期296-298,308,共4页
Journal of Hainan Medical University
基金
江苏省科技厅基础设施建设计划(BM2009903-KF19)~~
关键词
溃疡性结肠炎
兔
动物模型
Ulcerative colitis
Rabbit
Animal model