双酚A对人早孕绒毛组织IGF1、TGFβ1、ADAM12表达的影响

Effects of bisphenol A on the expression of IGF1、TGFβ1、ADAM12 in chorionic villus early in pregnancy

目的:研究双酚A(BPA)水平与人早孕胰岛素样生长因子1(IGF1),转化生长因子β1(TGFβ1),以及解整合素样金属蛋白酶12(ADAM12)表达的关系,探讨BPA对绒毛组织可能带来的影响。方法:以40例早期妊娠(6～8)周的新鲜绒毛组织进行体外培养,培养基中加入不同浓度的BPA:A组为1 000×10-6g/L,B组为500×10-6g/L,C组为1×10-6g/L,D组为对照组。收集培养第1天,培养第3天的培养液,采用ELISA方法检测收集的培养上清中TGFβ1、ADAM12及IGF1的分泌水平。结果:绒毛组织培养液第3天的TGFβ1、ADAM12的浓度降低(P<0.05)。第3天培养液中IGF1浓度变化不大(P>0.05)。结论:BPA对绒毛组织具有细胞毒性作用。

Objective：To study the effects of bisphenol A（BPA） on the expression of IGF1,TGFβ1,and ADAM12 in chorionic villus early in pregnancy.Methods：Villus samples collected from 40 early pregnancy cases（6～8 weeks） were cultured（in vivo） and treated with BPA at different concentrations.The concentrations of BPA was 1 000×10-6g/L for group A,500×10-6g/L for group B,1×10-6g/L for group C,respectively,group D was untreated control group.On the 1st,3rd day,IGF1、TGFβ1、ADAM12 concentrations of supernatant in the culture were measured by ELISA.Results：The concentrations of TGFβ1,ADAM12 were significantly decreased on day 3（P0.05）,but IGF1 showed little changes in concentrations（P0.05）.Conclusion：BPA is cytotoxic to villus during early pregancy.