NAC对日本血吸虫病小鼠肝组织中iNOS、NO、GSH和GSH-PX的影响

Effect of NAC on iNOS,GSH and GSH-PX in hepatic tissue of mice with schistosomiasis japonicum

目的:了解N-乙酰半胱氨酸(NAC)对日本血吸虫病小鼠肝组织中诱导型一氧化氮合酶(iN-OS)、一氧化氮(NO)、谷胱甘肽(GSH)及谷光甘肽-过氧化物酶(GSH-PX)的影响。方法:将小鼠随机分为正常组、模型组、预防a、b组和治疗a、b组,在感染同时给预防a、b组、感染后第6周给治疗a、b组分别以200 mg/kg和400 mg/kg NAC两种剂量灌胃,2次/d。正常组、模型组和预防组分别在第42天、56天时,治疗组在第56天时处死小鼠,取血清和肝脏,观察各组血清和肝组织中NO、GSH的含量及GSH-PX的活性、肝组织中iNOS的活性。结果:日本血吸虫肝病小鼠血清中NO的变化趋势和GSH与肝组织中的变化趋势不一致。预防a组和预防b组肝组织中iNOS活性和NO水平均介于正常组和模型组之间(P<0.05)。预防a组和治疗a组肝组织和血清中GSH水平均低于正常组和模型组(P<0.05)。NAC调节肝组织中iNOS、NO和GSH-PX等指标所达到的水平只与NAC的剂量有关(P<0.05),而与NAC的作用时间及NAC的剂量和作用时间的交互作用均没有关系(P>0.05)。NAC调节肝组织和血清中GSH的水平与NAC的剂量、作用时间和交互作用均有关(P<0.05)。结论:日本血吸虫病时,血清中NO和GSH等指标不能反映肝组织中含量。NAC可以改善日本血吸虫病小鼠肝组织内NO的含量i、NOS和GSH-PX活性。这可能是小剂量NAC减缓日本血吸虫病肝脏病变的早期效果最好的机制之一。但肝组织内NO的含量i、NOS和GSH-PX活性具有一个临界值。

Objective： To study the effect of N-acetylcysteine（NAC） on iNAS,NO,GSH and GSH-PX in hepatic tissue of mice with schistosomiasis japonicum.Methods： Mice were randomly divided into control group,model group,prevention group a,prevention group b,treatment group a and treatment group b.Gastric lavage with 200 mg/kg and 400 mg/kg NAC,twice per day were carried out to mice in prevention group a and prevention group b as infection,and mice in treatment group a and treatment group b 6 weeks after infection.Mice were scarified on the 42nd day in control group and model group,the 56th day in prevention group and treatment group.The serum and liver were collected.The indexes about hepatic pathological changes in serum and liver tissues were detected,including inducible nitric oxide synthase（iNOS）,nitric oxide（NO）,glutathione peroxidase（GSH-PX）,and reduced glutathione hormone（GSH）.Results： The concentration of NO and the activity of GSH in hepatic tissue showed different change trend.The activity of iNOS and NO level in prevention group were between the results of control group and model group.The GSH level of prevention group a and treatment group a was significant lower than control group and model group（P〈0.05）.The levels of iNOS,NO and GSH-PX were relative to dose of NAC,but there was no relationship between these indexes level and action duration of NAC,and interactivity of NAC action duration and dosage.While GSH level was associated with interactivity of NAC action duration and dosage,and NAC dosage.Conclusion： Serum levels of NO and GSH can′t be indices of content of NO and GSH in hepatic tissue.NAC can improve the NO content,and activity of iNOS and GSH-PX.That is possible mechanisms by which NAC at small dosage can alleviate hepatic damage due to Schistosoma japonicum.But there is critical value of NO,iNOS and GSH-PX.