高效液相色谱法测定葡萄中有机酸的含量

Determination of Organic Acids Content in Grape by HPLC

【目的】建立高效液相色谱法快速测定6种有机酸的方法。【方法】采用Ultimate C18色谱柱(4.6×250mm,5μm),流动相为磷酸盐缓冲溶液(pH2.0),流速0.5 mL/min,检测波长210 nm。【结果】建立了酒石酸、苹果酸、抗坏血酸、乳酸、乙酸、柠檬酸的高效液相色谱分析方法。测得相对标准偏差为1.38%～4.43%,检出限为0.012～2.291 mg/L,有机酸回收率为89.7%～105.8%。【结论】该方法分析速度快,具有较高的重现性及灵敏度,适用于葡萄中有机酸的测定分析。

【Objective】A method for rapid determination of 6 organic acids with high performance liquid chromatography（HPLC） was developed.【Method】The wavelength of 210 nm were detected by use of a Ultimate C18 column（4.6×250 mm,5μ m）,using phosphate buffer solution（pH2.0） as mobile phase at the flow rate of 0.5 mL/min .【Result】A method of HPLC determination for Tartaric acid,Malic acid,Ascorbic acid,Lactic acid,Acetic acid and Citric acid was developed.The relative standard deviations（RSD） was 1.38%-4.43% and the limits of detection was 0.012-2.291 mg/L.The recovery rate of the organic acids were 89.7%-105.8%.【Conclusion】The method was fast,reproducible and practical for the routine analysis of organic acids in grape.