大肠杆菌感染后栉孔扇贝血淋巴中7种酶活力的变化

ACTIVITY CHANGE OF SEVEN ENZYMES IN HAEMOLYMPH IN SCALLOP Chlamys farreri AFTER CHALLENGE WITH Escherichia coli

于１９９８年１１月对栉孔扇贝注射大肠杆菌，分别于１，１５，３０ ｈ 测定了栉孔扇贝血 清和血细胞中参与免疫防御的水解、氧化和抗氧化酶的活力。结果表明，大肠杆菌激发后， 血清实验组的ＡＣＰ活力在１ｈ时显著高于对照组，１５ｈ和３０ｈ时极显著地高于对照组；血 细胞实验组的ＡＣＰ活力仅在１５ｈ时极显著地高于对照组。 血清和血细胞实验组与对照组 的ＡＫＰ活力除了血清３０ｈ时差异极显著外均为差异不显著。 除了血细胞实验组的溶菌酶 活力在１５ｈ时极显著地高于对照组外其他组均差异不显著。血清实验组的ＰＯ活力在１， １５，３０ｈ时均极显著地高于对照组；血细胞中无ＰＯ活力。血清实验组与对照组的ＭＰＯ活力 均差异不显著；而血细胞中却均差异极显著。血清中ＳＯＤ活力均差异不显著；血细胞中 ＳＯＤ活力则在１５ｈ和３０ｈ时极显著地高于对照组。 血清中ＣＡＴ活力在１ｈ时极显著地高 于对照组，而在１５ｈ和３０ｈ时则显著地低于对照组；血细胞中ＣＡＴ活力在１ｈ和１５ｈ时均 极显著地高于对照组，而在３０ｈ时则显著低于对照组。

Seven kinds of hydrolytic, oxidant and antioxidant enzymes participating in the immune defense in the haemolymph of Chlamys farreri were assayed at 1, 15 and 30 h after challenge with Escherichia coli in November, 1998. The results show that the ACP activities of experimental groups in serum were much higher than that of control groups at 1, 15 and 30 h. The ACP activities of experimental groups in haemocytes were much higher than that of control groups only at 15 h. The differences of AKP activities of experimental and control groups in serum and haemocytes were not obvious except the group of serum at 30 h. The differences of lysozyme activities of experimental and control groups in serum and haemocytes were not notable except the group of haemocytes at 15 h. The PO activities of experimental groups in serum were much higher than that of control groups at l, 15 and 30 h. There were no activities of PO in haemocytes. The differences of MPO activities of experimental and control groups in serum were not notable, but the differences in haemocytes were very significant. The differences of SOD activities in serum were not notable, but the differences in haemocytes at 15 and 30 h were very notable. The CAT activities of experimental groups in serum or in haemocytes were much higher than that of control groups at 1 h or at 1 and 15 h.