摘要
于1998年11月对栉孔扇贝注射大肠杆菌,分别于1,15,30 h 测定了栉孔扇贝血 清和血细胞中参与免疫防御的水解、氧化和抗氧化酶的活力。结果表明,大肠杆菌激发后, 血清实验组的ACP活力在1h时显著高于对照组,15h和30h时极显著地高于对照组;血 细胞实验组的ACP活力仅在15h时极显著地高于对照组。 血清和血细胞实验组与对照组 的AKP活力除了血清30h时差异极显著外均为差异不显著。 除了血细胞实验组的溶菌酶 活力在15h时极显著地高于对照组外其他组均差异不显著。血清实验组的PO活力在1, 15,30h时均极显著地高于对照组;血细胞中无PO活力。血清实验组与对照组的MPO活力 均差异不显著;而血细胞中却均差异极显著。血清中SOD活力均差异不显著;血细胞中 SOD活力则在15h和30h时极显著地高于对照组。 血清中CAT活力在1h时极显著地高 于对照组,而在15h和30h时则显著地低于对照组;血细胞中CAT活力在1h和15h时均 极显著地高于对照组,而在30h时则显著低于对照组。
Seven kinds of hydrolytic, oxidant and antioxidant enzymes participating in the immune defense in the haemolymph of Chlamys farreri were assayed at 1, 15 and 30 h after challenge with Escherichia coli in November, 1998. The results show that the ACP activities of experimental groups in serum were much higher than that of control groups at 1, 15 and 30 h. The ACP activities of experimental groups in haemocytes were much higher than that of control groups only at 15 h. The differences of AKP activities of experimental and control groups in serum and haemocytes were not obvious except the group of serum at 30 h. The differences of lysozyme activities of experimental and control groups in serum and haemocytes were not notable except the group of haemocytes at 15 h. The PO activities of experimental groups in serum were much higher than that of control groups at l, 15 and 30 h. There were no activities of PO in haemocytes. The differences of MPO activities of experimental and control groups in serum were not notable, but the differences in haemocytes were very significant. The differences of SOD activities in serum were not notable, but the differences in haemocytes at 15 and 30 h were very notable. The CAT activities of experimental groups in serum or in haemocytes were much higher than that of control groups at 1 h or at 1 and 15 h.
出处
《海洋科学》
CAS
CSCD
北大核心
1999年第5期40-41,共2页
Marine Sciences
基金
国家攀登计划B资助项目!PDB6-6-3号
关键词
栉孔扇贝
大肠杆菌感染
血淋巴
酶活力
Escherichia coli, Chlamys farreri, Haemolymph, Enzyme activity