碳纳米纤维糊电极电化学发光法测定甲硫哒嗪

Electrochemiluminescence Determination of Thioridazine Based on Carbon Nanofiber Paste Electrode

制备了基于碳纳米纤维糊电极(CFPE)的新型电化学发光传感器。运用电化学方法对CFPE进行了表征,并考察了三联吡啶钌和甲硫哒嗪在此电极上的电化学行为和电化学发光行为。结果表明,该电极表现出很好的电化学活性和电化学发光响应。基于甲硫哒嗪对三联吡啶钌电化学发光的增强作用,建立了测定甲硫哒嗪的电化学发光新方法。实验考察了缓冲溶液的pH值、浓度以及发光试剂三联吡啶钌浓度对体系电化学发光的影响。在优化的实验条件下,甲硫哒嗪浓度在2～40μmol/L范围内与其增敏的三联吡啶钌电化学发光强度呈线性关系(R=0.998),检出限为0.5μmol/L(S/N=3)。对15μmol/L的甲硫哒嗪平行测定11次,其相对标准偏差为1.12%。本方法已成功用于尿样中甲硫哒嗪的测定,其回收率为98.7%～105.4%,结果令人满意。

A carbon nanofiber paste electrode（CFPE） was employed for fabrication of electrochemilumunescence（ECL） sensor.The performance of CFPE was characterized by cyclic voltammetry.The electrochemical and ECL behaviors of Ru（bpy）2＋3 and thioridazine（TR） were investigated.The electrode showed an excellent electrochemical activity and ECL response.Based on the fact that the light emission of the ECL sensor could be enhanced by TR dramatically,a new method has been established for the determination of TR.The effects of pH value and concentration of buffer,Ru（bpy）2＋3 were studied respectively.Under the optimum experimental conditions,the ECL sensor exhibited excellent linear response to TR ranging from 2 to 40 μmol/L（R=0.998） and a low detection limit of 0.5 μmol/L（S/N=3）.The relative standard deviation was 1.1% for 15 μmol/L TR（n=11）.The established method was successfully applied for the determination of TR in urine sample with satisfactory results,and the recovery was between 98.7% and 105.4%.