摘要
目的了解耐万古霉素屎肠球菌(VREFm)的基因存在状况,有利于临床治疗选药。方法依据2008年美国临床实验室标准化研究所推荐的纸片扩散法和琼脂稀释法,对VREFm进行药敏试验;PCR方法检测万古霉素和其他耐药基因的情况。结果万古霉素、替考拉宁、红霉素、青霉素G、四环素、利福平、克林霉素和高浓度庆大霉素均耐药,呋喃妥因、左氧氟沙星和利奈唑胺均为敏感,万古霉素MIC值为128μg/ml,基因表型为VanA,还检出TEM型β-内酰胺酶基因、ermB和mefA型红霉素耐药基因、aac(6′)-aph(2″)双功能酶编码基因和ant(6)-Ⅰ核苷酸转移酶编码基因。结论耐万古霉素屎肠球菌存在多种耐药基因。
OBJECTIVE To assess the characterization of resistant related genes in vancomycin-resistant Enterococcus faecium（VREF）.METHODS According to the Clinical Laboratory Institution Standards（CLIS） in 2008,K-B disc diffusion method and agar dilution method were used to determine the susceptibility.VREFm was then subjected to PCR for resistance related genes.RESULTS The susceptibility test showed it was sensitive to nitrofurantoin,levofloxacin and linezolid,but resistant to the others.MIC for vancomycin was 128ug/ml.Resistant related genes,including VanA type vancomycin resistant gene,TEM β-lactamases gene,erm and mefA erythromycin resistance genes,aac（6′）-aph（2″） and ant（6）-Ⅰ aminoglycoside resistance genes,were amplified successfully.CONCLUSION VREF is a bacterium carrying multidrug-resistant related genes.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第9期1747-1748,共2页
Chinese Journal of Nosocomiology
关键词
万古霉素
耐药性
屎肠球菌
表型
基因型
Vancomycin
Drug resistance
Enterococcus faecium
Phenotype
Genotype
