摘要
目的探讨实时荧光聚合酶链反应(PCR)在检测解脲脲支原体(Uu)中的临床意义。方法对278例未经治疗的门诊就诊者和35例确诊为Uu感染的患者经抗菌药物应用两周停药1周后分别采用实时荧光PCR和培养技术进行Uu的检测。结果 278例首次就诊者经实时荧光PCR检测Uu阳性125例,阳性率44.96%,培养阳性113例,阳性率40.65%,两者比较差异无统计学意义,35例确诊为Uu患者经抗菌药物治疗后停药1周,采用实时荧光PCR检测阳性12例,阳性率34.29%,但病原体含量要远远低于用药初期,35例患者经培养阳性5例,阳性率14.29%;两者比较差异有统计学意义(P<0.05)。结论实时荧光PCR在判断是否存在Uu感染时要较培养法灵敏,对经抗菌药物治疗停药后判断是否治愈时培养法要较荧光PCR更为准确。
OBJECTIVE To explore the clinical significance in detection of Ureaplasma urealyticumwith Real-time PCR.METHODS A total of 278 patients untreated and 35 cases were diagnosed as Uu infections after discontinuation of antibiotics for two weeks were sampled and tested by real-time PCR and cultivation technique,fluorescence Uu detection.RESULTS A total of 278 patients were the first real-time PCR detection fluorescent Uu positive rate of 125(44.96%),cultivate positive 113 cases were compared 40.65% both were not statistically significant(P〉0.05),35 patients were diagnosed by antibiotics Uu patients after 1 week withdrawal by real-time PCR,12 cases were positive(34.29%) but the pathogen content than medication,35 patients with early in 5 cases,the positive rate of 14.29%.There was statistical differences(P〈0.05).CONCLUSION Real-time PCR is more sensitive than the cultivate method when judging whether exists the Uu infection,but the cultivate method is more accurate than real-time PCR when judging if it cures after the withdrawal.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第9期1931-1933,共3页
Chinese Journal of Nosocomiology