影响猪胚胎玻璃化冷冻的若干因素

Some factors for porcine embryos vitrification

以广西巴马小型猪为供体,采用超数排卵技术,采集5~6日龄的胚胎（囊胚/桑椹胚）,比较2种冷冻方法、胚胎承载工具、透明带处理和冷冻胚胎移植受体对猪胚胎冷冻效果的影响。结果表明,2种冷冻方法的冷冻效果没有显著差异;GMP法能显著提高冷冻胚胎存活率（83.8%vs 77.6%,P〈0.05）和囊胚细胞数（47.5 vs 53.1,P〈0.05）;以0.5%链蛋白酶10 s处理透明带,虽然对猪胚胎存活率没有显著影响,但能显著提高囊胚细胞数（60.1vs 46.6,P〈0.01）;以地方猪种（枫泾母猪）为冷冻胚胎移植受体能显著提高妊娠率和胚胎效率（P〈0.01）。

The purpose of this study was to optimize the procedure for cryopreservation of porcine embryos by vitrification.5-6 day-embryos（blastocyst/morula）were collected from superovulated Bama mini-pigs（sows/gilts）.Different cryopreservation methods,cryopreservation tools,thining of zona pellucida（ZP） and recipient breeds were compared.The results were as follows：there were no significant different between two freezing method in embryo survival rate and cell number;the GMP vitrification method was more suitable and efficient in cryopreservation of pig embryos,the embryo survival rate（83.8% vs 77.6%） and blastocyst cell number（53.1 vs 47.5） were significantly higher than the OPS method（P0.05）;there were no significant differences in vitro survival rate by thinning the ZP（0.5% pronase,10 s） after warming but significantly improved the blastocyst cell number in surviving blastcysts（60.1% and 46.6%,P0.01）;the local pig breeds（Fengjing sows） are more suitable as recipients for vitrified/warmed blastcysts in the pregnancy rate and embryos efficiency（P0.01）.