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EBNA3C combines with Gemin3 and up-regulates its expression

EBNA3C combines with Gemin3 and up-regulates its expression
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摘要 Objective To investigate the combination of Epstein-Barr virus nuclear protein 3C (EBNA3C) with Gemin3 and its effect on Gemin3 expression. Methods Co-immunoprecipitation, GST pull-down and immunofluorescent assay were used to determine the combination of EBNA3C and Gemin3 and their combining domain. Stable EBNA3C knockdown cell lines were made by lentivirus-delivered small hairpin RNA and then puromycin selection. Western blot was used to check the effect of EBNA3C on Gemin3 expression. Results EBNA3C and Gemin3 combined with each other in vivo and in vitro through their C-terminals. EBNA3C up-regulated Gemin3 gene expression. Conclusion EBNA3C forms complex with Gemin3 and up-regulates its expression. Objective To investigate the combination of Epstein-Barr virus nuclear protein 3C （EBNA3C） with Gemin3 and its effect on Gemin3 expression. Methods Co-immunoprecipitation, GST pull-down and immunofluorescent assay were used to determine the combination of EBNA3C and Gemin3 and their combining domain. Stable EBNA3C knockdown cell lines were made by lentivirus-delivered small hairpin RNA and then puromycin selection. Western blot was used to check the effect of EBNA3C on Gemin3 expression. Results EBNA3C and Gemin3 combined with each other in vivo and in vitro through their C-terminals. EBNA3C up-regulated Gemin3 gene expression. Conclusion EBNA3C forms complex with Gemin3 and up-regulates its expression.
作者 Qian Wang Dan Zhou Yi Guo
机构地区 Department of Laboratory Medicine Department of Obstetrics and Gynecology Department of Gynecology
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2011年第6期697-708,共12页 China Journal of Modern Medicine
关键词 EBNA3C Gemin3 gene expression regulation gene knock down EBNA3C, Gemin3, gene expression regulation, gene knock down
分类号 Q257 [生物学—细胞生物学]
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中国现代医学杂志
2011年 第6期

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