微波萃取快速分析补骨脂中补骨脂素和异补骨脂素

Rapid analysis of psoralen and isopsoralen in Psoralea corylifolia by microwave-assisted extraction

目的:建立简便、快速的微波萃取法,用于提取补骨脂中有效成分补骨脂素、异补骨脂素。方法:以补骨脂素和异补骨脂素的提取率为考察指标,对微波萃取的参数进行单因素试验,以选择最佳的萃取条件,并与传统提取方法比较。结果:以甲醇为溶剂,微波功率400 W,液固比400 mL.g-1的条件下萃取3 m in,2种有效成分的提取量便可达到最高,而传统的索氏提取、回流及超声提取法则需要几十分钟甚至几小时。结论:微波萃取大大节省了提取时间、提高了提取效率,为补骨脂中补骨脂素、异补骨脂素的分离分析提供了一种简便快速的新手段。

Objective：To establish a convenient and rapid method of microwave-assisted extraction（MAE） for the extraction of psoralen and isopsoralen in Psoralea corylifolia.Method： The effects of various parameters on the MAE were investigated with several single-factor tests,and compared with the traditional extraction.Result： The optimal conditions of MAE were as follows： the extractine time was 3 min,and the ratio of solvent to material was 400 mL·g-1 by using methanol as the solvent.Compared with soxhlet extraction,heat reflux extraction,and ultrasound-assisted extraction,MAE needed only 3 min to give the highest yield of psoralen and isopsoralen,while the other extraction methods needed several hours and gave lower yield.Conclusion： The proposed method has greatly shortened extraction time and improved extraction yield,so it provides a neotype,simple and rapid measure for the separation of psoralen and isopsoralen from P.corylifolia.