摘要
目的:提出一种快速、无损检测白芍药材中芍药苷、芍药内酯苷及水分的新方法。方法:运用偏最小二乘法(PLS)建立近红外光谱(NIR)与芍药苷、芍药内酯苷和水分测定值之间的多元校正模型,对未知样品进行含量预测。结果:校正模型相关系数(R2)分别为0.938,0.943,0.976。验证集预测平均相对偏差分别为6.5%,0.23%,3.8%。结论:近红外光谱法具有分析速度快、预测结果准确、不破坏样品和不污染环境等优点,而且不需要对样品进行复杂繁琐的前处理,适合对组成复杂的中药进行快速分析,可用于白芍配方颗粒原料药材的质量监控。
Objective:To develop a novel method to determine fastly and nondestructively the content of paeoniflorin,albiflorin and moisture in Radix Paeoniae Alba with near infrared diffuse reflection spectroscopy.Method: Multivariate calibration models based on PLS algorithm were developed to correlate the spectra and the corresponding values determined by the reference method.Result: The corelation coefficients(R2) of the calibration models were 0.938,0.943 and 0.976,and the prediction average relative deviation for paeoniflorin,albiflorin and moisture content were 6.5%,0.23% and 3.8%,respectively.Conclusion: The NIRS determination method is rapid,accurate,nondestructive and non-pollution.It can dispose the samples without complicated pretreatment.It is qualified to analyze rapidly traditional Chinese medicine whose components are complex.NIRS can be used to control the quality of herbal material of paeony formula granule.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第9期1162-1167,共6页
China Journal of Chinese Materia Medica
关键词
近红外漫反射光谱法
白芍配方颗粒
白芍药材
质量控制
near infrared diffuse reflection spectroscopy; paeony formula granule; radix paeoniae alba; quality control;