镰形棘豆对SMMC-7721肝癌细胞增殖及MMP-2表达的影响

Influences of Oxytropis falcata on proliferation of SMMC-7721 Hepatocarcinoma cells and expression of MMP-2

目的:观察镰形棘豆对人肝癌细胞SMMC-7721的增殖及其基质金属蛋白酶-2(MMP-2)分泌量的影响。方法:以不同质量浓度(25,50,100,250,500 mg.L-1)的镰形棘豆挥发油、总多糖、总黄酮、总生物碱和总皂苷部位处理SMMC-7721细胞24 h后,采用MTT法检测各部位对SMMC-7721细胞增殖的抑制作用;采用ELISA法测定挥发油部位和总黄酮部位处理SMMC-7721肝癌细胞24 h后细胞上清中MMP-2的分泌量;Real-time PCR法检测处理24 h后MMP-2 mRNA的转录情况。结果:MTT实验结果表明,挥发油和总黄酮部位抑制SMMC-7721细胞的增殖,并表现出一定的浓度依赖性。ELISA实验表明挥发油部位显著性地抑制MMP-2分泌量;Real-time PCR实验表明挥发油和总黄酮均显著抑制MMP-2 mRNA的表达。结论:镰形棘豆挥发油和总黄酮部位抑制人肝癌细胞SMMC-7721的增殖活性,可能与其下调MMP-2的分泌量和转录水平有关。

Objective：To investigate the influences of extracts of Oxytropis falcata on proliferation of SMMC-7721 cells and expression of MMP-2（matrix metalloproteinase-2）.Method： SMMC-7721 cells were treated for 24 h with five fractions obtained from O.falcata in different concentrations.Inhibition on proliferation of the SMMC-7721 cells was assessed by MTT method.Secretion of MMP-2 was measured by ELISA in the supernatant of SMMC-7721 cells treated with fractions of essential oil and total flavonoids for 24 h.Transcription of mRNA of MMP-2 was detected by Real-time PCR.Result： In MTT assay,essential oil and total flavonoids showed potential antiproliferative activity on SMMC-7721 in a concentration dependent manner.Data of ELISA showed that fraction of essential oil suppressed secretion of MMP-2 significantly.Results of Real-time PCR indicated that both essential oil and total flavonoids restrained expression of mRNA of MMP-2.Conclusion： It suggested that essential oil and total flavonoids of O.falcata inhibit proliferation of SMMC-7721 cells through down-regulating secretion and expression of MMP-2 in cells.However,further experiments are necessary to carry out to investigate the potential mechanism.