灵芝孢子油通过下调miR-21促进人肺腺癌SPC-A1细胞凋亡

Glossy ganoderma spore oil promotes apoptosis of human lung adenocarcinoma SPC-A1 through downregulation of miR-21

目的:通过检测灵芝孢子油对人肺腺癌细胞SPC-A1增殖、凋亡与miR-21及其靶基因表达的影响,探讨灵芝孢子油的抗肿瘤机制。方法:灵芝孢子油处理人肺腺癌细胞SPC-A1 24 h以及48 h后,采用CCK-8法检测其对细胞增殖的影响,倒置相差显微镜观察细胞形态学变化,流式细胞术检测细胞凋亡,Real-time PCR检测miR-21及其靶基因PTEN和PDCD4的表达。结果:灵芝孢子油剂量、时间依耐性地抑制人肺腺癌SPC-A1细胞的增殖;当灵芝孢子油体积分数达到0.2%时,细胞形态发生明显变化;Annexin-V/PI双染检测提示,灵芝孢子油在低浓度下就可促进细胞凋亡;灵芝孢子油显著下调SPC-A1细胞中miR-21的表达并相应上调PTEN以及PDCD4基因的表达。结论:灵芝孢子油能显著抑制人肺腺癌细胞SPC-A1细胞增殖,引起细胞形态学改变。通过下调miR-21的表达,上调抑癌基因PTEN以及PDCD4的表达,从而促进肺癌细胞凋亡,达到抗肿瘤的作用。

Objective：To investigate the effects of glossy ganoderma spore oil on the proliferation,apoptosis,expression of miR-21 and its target genes of human lung adenocarcinoma SPC-A1 cell line,and to explore its possible mechanism.Method： The SPC-A1 cells were treated with glossy ganoderma spore oil for 24 and 48 hours.The inhibition growth efficacy was determined using cell count kit（CCK-8）.Cell morphological changes were observed by light microscopy.Cell apoptosis was analyzed by flow cytometry.The expression of miR-21,PTEN and PDCD4 were determined by Real-time PCR.Result： Glossy ganoderma spore oil concentration-dependently inhibited the SPC-A1 cell’s proliferation.When the concentration of glossy ganoderma spore oil attained to 0.2%,the cells’ morphology changed obviously.Glossy ganoderma spore oil could induce the apoptosis of SPC-A1 cells at low concentration.Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly.Conclusion： glossy ganoderma spore oil could inhibit the proliferation obviously and cause the changes of cell morphology.Furthermore,glossy ganoderma spore oil induced apoptosis of SPC-A1 cell through down-regulating the expression of miR-21 and up-regulating tumor suppressors.