胶基烟草制品中烟草特有亚硝胺(TSNAs)的分析

Analysis of Tobacco-specific N-nitrosamines in Gum-based Tobacco Products

为检测胶基烟草制品中的烟草特有亚硝胺（TSNAs）,建立了一种同时测定4种TSNAs的液相色谱串联质谱方法。以NNN-d4,NNK-d4,NAB-d4,NAT-d4为内标,以0.05 mol/L盐酸为提取溶液,通过HLB小柱固相萃取、Agilent Poroshell 120 SB-C18柱分离和多离子监测模式检测了实际样品中的TS-NAs。结果表明：①TSNAs的定量限为0.026~0.076 ng/mL,回收率为80.00%~126.86%,相对标准偏差（RSD）为2.99%~8.32%;②样品中总TSNAs的范围为5.13~13.76 ng/g。其中,NNN与NAT含量最高,占总量的91.23%~95.71%。该方法适用于胶基烟草制品中TSNAs的分析。

A method based on liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of four tobacco-specific N-nitrosamines（TSNAs） in gum-based tobacco products.Using NNN-d4,NNK-d4,NAB-d4,NAT-d4 as internal standards,samples were successively extracted with 0.05 mol/L HCl aqueous solution,cleaned up by HLB solid phase extraction cartridge,separated by an Agilent Poroshell 120 SB-C18 column,and analyzed by a tandem mass detector under multi-reaction monitoring（MRM） scan mode.The results showed that： 1） the limits of quantification of TSNAs varied from 0.026 to 0.076 ng/mL,the recoveries ranged from 80.00% to 126.86% and relative standard deviation（RSD） from 2.99% to 8.32%;2） the total concentration of TSNAs ranged from 5.13 to 13.76 ng/g.The concentrations of NNN and NAT were much higher and their sum accounted for 91.23% to 95.71% of total TSNAs yields.The method is fit for the simultaneous analysis of TSNAs in gum-based tobacco products.