NaYF_4：Yb/Er-碳纳米管功能纳米材料的制备、表征及其在肿瘤细胞的靶向检测和治疗中的应用

Functional nanomaterials of NaYF_4:Yb/Er-MWCNT: Synthesis, characterization and applications in targeted diagnosis and therapy of tumor cell

将叶酸分子(FA)和2,3-二巯基丁二酸(DMSA)修饰的稀土上转换发光纳米粒子NaYF4:Yb/Er通过酰胺键偶联在多壁碳纳米管(MWCNT)的表面,得到NaYF4:Yb/Er-MWCNT-FA功能化复合纳米材料,并通过透射电子显微镜(TEM)、X射线衍射(XRD)、紫外-可见吸收光谱(UV-vis)、荧光光谱(PL)和共聚焦激光扫描显微镜等手段表征了其形貌、结构、发光性能和靶向成像性能.共聚焦激光扫描显微镜结果表明,相对于正常的HLF细胞,所制备的复合材料能够靶向检测叶酸受体高表达的宫颈癌Hela细胞.此外,将阿霉素进一步通过"ππ"堆垛吸附在此复合材料后,该载药体系具有明显的抗肿瘤活性,能够实现对肿瘤细胞的一步检测和治疗.

NaYF4：Yb,Er-MWCNT-FA composite nanomaterials were obtained by conjugating the carbon nanotubes with folic acid and 2,3-dimercaptosuccinic acid functionalized NaYF4：Yb,Er nanoparticles via an amide bond. Their morphology, structures, fluorescent and target imaging properties were characterized by means of TEM, XRD, UV-Vis, PL and confocal laser scanning microscopy （CLMS）. The results of CLMS demonstrated that the functional composites had a high effectiveness to detect cancerous cell lines overexpressing the folate receptor （Hela cell） from normal cells （HLF cell） by tracing the fluorescence imaging with high fidelity. Furthermore, DOX was successfully loaded to the prepared NaYF4：Yb,Er-MWCNT-FA nanomaterials mainly through noncovalent π-π interactions, which had obvious antitumor activities and achieved targeted diagnosis and therapy of tumor cell simultaneously.