用流式细胞术分析双氢青蒿素对杜氏利什曼原虫DNA的作用

EFFECT OF DIHYDROARTEMISININ ON LEISHMANIA DONOVANI PROMASTIGOTES DNA ANALYSED BY FLOW CYTOMETRY

应用流式细胞术（ Ｆ Ｃ Ｍ ）检测分析了双氢青蒿素对杜氏利什曼原虫前鞭毛体 Ｄ Ｎ Ａ 的作用。４ 个不同浓度（１７７×１０－ ４，３５３×１０－ ４，７１×１０－ ４，１４１×１０４ｍ ｏｌ／ Ｌ）的药物对虫体 Ｄ Ｎ Ａ 均有抑制作用，用药组虫体 Ｄ Ｎ Ａ 含量的荧光分布随着药物浓度的升高而减弱，抑制率分别为 ３２３％ ，３２６％ ，８６４％ ，８９２％ ，与对照组相比有显著性差异（ Ｐ＜ ００５）。后两个用药组与前两个用药组抑制率有显著性差异（ Ｐ＜ ０００５）。本研究结果与光镜观察、３ Ｈ Ｔｄ Ｒ

In the paper, the effect of dihydroartemisinin on Leishmania donovani promastigotes DNA is analysed by flow cytometry. L.donovani promastigotes DNA was inhibited by dihydroartemisinin under four different concentrations (1.77×10 -4 , 3.53×10 -4 , 7.1×10 -4 , 14.1×10 -4 mol/L). In experiment groups, the fluorescent distribution of DNA content in L.donovani promastigotes decreased when drug concentration increased and with inhibition rates of 32.3%, 32.6%, 86.4% and 89.2% respectively. There were significant differences between the former two concentration groups and the latter two concentration groups ( P <0.005). Besides, there were also significant differences between 4 experiment groups and control group ( P <0.05). The result was coincident with that of microscopy observation and 3 H TdR marking experiment.