信号转导与转录激活子3、nm23、CXCR4在原发性乳腺癌组织中的表达及其意义

Expression of STAT3, nm23, and CXCR4 in primary breast cancer and its significance

目的探讨信号转导与转录激活子3（STAT3）、nm23、CXCR4在原发性乳腺癌中的表达及其相互关系。方法采用免疫组织化学sP法检测82例乳腺良性疾病和368例原发性乳腺癌中nm23、CXCR4蛋白的表达，应用逆转录一聚合酶链反应（RT—PCR）方法测定82例乳腺良性疾病和368例原发性乳腺癌中STAT3mRNA的表达水平，分析STAT3与nm23、CXCR4表达的相互关系。结果乳腺良性疾病组织STAT3mRNA相对表达量0．10±0．02，乳腺癌组织STAT3mRNA相对表达量0．48±0．09，两者差异有统计学意义（P〈0．01）。乳腺原位癌和浸润性癌STAT3mRNA相对表达量分别为0．32±0．05、0．95±0．18，两者差异有统计学意义（P〈0．01）。无腋淋巴结转移的乳腺癌和有腋淋巴结转移的乳腺癌STAT3mRNA相对表达量分别为0．38±0．06、0．88±0．15，两者比较差异有统计学意义（P〈0．01）。乳腺良性疾病组织nm23蛋白表达阳性率明显高于乳腺癌组织，乳腺原位癌nm23蛋白表达阳性率明显高于浸润性癌，无腋淋巴结转移的乳腺癌nm23蛋白表达阳性率明显高于有腋淋巴结转移的乳腺癌。乳腺良性疾病组织CXCR4蛋白表达阳性率明显低于乳腺癌组织，乳腺原位癌CXCR4蛋白表达阳性率明显低于浸润性癌，无腋淋巴结转移的乳腺癌CXCR4蛋白表达阳性率明显低于有腋淋巴结转移的乳腺癌。STAT3与nm23表达呈明显负相关，STAT3与CXCR4表达呈明显正相关。结论nm23、CXCR4蛋白与原发性乳腺癌的浸润和转移密切相关，STAT3信号通路可能通过作用于nm23、CXCR4调控乳腺癌的侵袭和转移。

Objective To explore the expression of STAT3, nm23, CXCR4 in primary breast cancer and their correlation. Methods The expression of nm23, and CXCR4 proteins in 82 cases of breast benign disease and 368 cases of primary breast cancer was detected by using SP immunohistochemical staining. Reverse transcription-polymerase chain reaction （RT-PCR） was applied to detect STAT3 mRNA expression in breast benign disease and primary breast cancer. The correlation between STAT3 and nm23, CXCR4 was also analyzed. Results The average level of STAT3 mRNA expression in breast benign disease and breast cancer was 0. 10 ± 0. 02 and 0. 48 ±0. 09 respectively （ P 〈 0. 01 ）. The average level of STAT3 mRNA expression in breast cancer in situ and invasive breast cancer was 0. 32 ± 0. 05 and 0. 95 ± 0. 18 respectively （P 〈 0. 01 ）. The average level of STAT3 mRNA expression in breast cancer without axillary lymph node metastases and with axillary lymph node metastases was 0. 38± 0. 06 and 0. 88 -±0. 15 respectively （P 〈0. 01 ）. The positive rate of nm23 protein in breast benign disease was obviously higher than that in breast cancer. The positive rate of nm23 protein in breast cancer in situ was significantly higher than that in invasive breast cancer. The positive rate of nm23 protein in breast cancer without axillary lymph node metastases was markedly higher than that in breast cancer with axillary lymph node metastases. The positive rate of CXCR4 protein in breast benign disease was obviously lower than that in breast cancer. The positive rate of CXCR4 protein in breast cancer in situ was significantly lower than that in invasive breast cancer. The positive rate of CXCR4 protein in breast cancer without axillary lymph node metastases was markedly lower than that in breast cancer with axillary lymph node metastases. The expression of STAT3 and nm23 had an obviously negative correlation （ P 〈 0. 05 ）. However, there was an obviously positive correlation between the expression of STAT3 and CXCR4 （ P 〈 0. 05 ）. Conclusion nm23, and CXCR4 proteins play an important role in the invasiveness and metastasis of primary breast cancer. The ability to invasiveness and metastasis of primary breast cancer was perhaps controlled by STAT3 signal pathway with impact on nm23 and CXCR4.