趋化因子2对乳腺癌细胞MCF-7趋化活性及趋化因子5mRNA表达的影响

Effect of CCL2 on the expression of CCLs mRNA and the chemotactic activity in MCF-7 ceils

目的观察趋化因子2（CCL2）对MCF-7表达趋化因子5（CCL2）mRNA的影响，并观察CCL2对MCF-7趋化活性的影响。方法使用不同浓度的ccL2作用于MCF-7细胞，通过实时荧光定量聚合酶链反应（RTFQ—PCR）测定不同时间CCL，mRNA的表达，并使用趋化小室法检测CCL2作用下MCF-7趋化活性的改变。结果当外源性CCL2浓度为200IXg／L时，MCF-7中CCkmRNA相对表达量最大（15．22±2．32）（P〈0．01），随着作用时间的延长，CCL2mRNA表达量增加；MCF-7的趋化活性与CCL2浓度呈正相关，当CCL2浓度为300μg／L时，穿膜细胞数最多（88．00±11．53）（P〈0．01）；MCF-7的趋化活性与CCL2作用时间呈正相关，当CCL2作用时间为30h时，穿膜细胞数最多（81．00±9．54）（P〈0．05）。结论加入外源性CCL2，MCF-7中CCL5mRNA表达量增加，MCF-7趋化活性增强。

Objective To observe the effects of chemokine 2 （ CCL2 ） on the expression of chemo- kine 5 （ CCL5 ） mRNA and chemotactic activity of MCF-7 cells. Methods MCF-7 cells were treated with different concentrations of CCL2 , the expression of CCL5 mRNA was detected by using real-time fluorescence quantitative polymerase chain reaction （ RTFQ-PCR）, and the chemotactic activity of MCF-7 was measured by using ehemotaxis chamber method. Results When the concentration of exogenous CCL2 was 200μg/L, the MCF-7 cells expressed the highest CCL5 mRNA （ 15.22 ± 2. 3, P 〈 0. 01 ）. With the prolongation of CCL2 action time, the expression levels of CCL5 mRNA were increased. There was a positive correlation between the chemotactic activity of MCF-7 cells and the concentration of CCL2. When the concentration of exogenous CCL2 was 300 p,g/L, the number of penetrating cells was the greatest （ 88. 00 ± 11.53, P 〈 0. 01 ）. With the prolongation of CCL2 action time, the chemotactie activity of MCF-7 cells was enhanced. When the action time was 30 h, the number of penetrating cells was the greatest （ 81.00 ± 9. 54, P 〈 0. 05 ）. Conclusion Exogenous CCL2 could increase the expression of CCLs mRNA and the ehemotactie activity of MCF-7 cells.