摘要
目的观察二硫代氨基甲酸吡咯烷(PDTC)和甲氨蝶呤(MTX)对人骨肉瘤MG_63细胞凋亡和Livin表达的影响。方法体外培养骨肉瘤MG-63细胞株,用0、50、100、200和400μmol/L的PDTC和MTX作用于骨肉瘤MG_63细胞24、48和72h后,用噻唑蓝(MTT)比色法检测骨肉瘤MG-63细胞的增殖活性,用流式细胞仪测细胞的凋亡率,用Westernblot检测各组细胞的Livin蛋白表达水平。结果各组骨肉瘤MG63细胞的增殖活性随着时间增加降低,同时细胞的凋亡率随着时间增加(P〈0.05),各组细胞中Livin蛋白的表达明显降低(P〈0.05)。结论PDTC能提高MTX对骨肉瘤MG-63细胞的凋亡,其机制可能与下调Livin表达,阻断了Lvin介导的抗凋亡途径有关。
Objective To detect the effect of pyrrolidine dithiocarbamate (PDTC) and methotrexate (MTX) on apoptosis and expression of livin of human osteosarcoma MG-63 cells. Methods MG-63 cells were cultured in vitro. At 24, 48 and 72 h after treatment of PDTC and MTX at different concentrations (0, 50, 100,200 and 400 ixmol/L) , MTT assay was used to observe the growth inhibition of MG-63 cells. The apoptosis was assessed by flow cytometry. ern blotting. Results When PDTC and MTX were The protein expression of livin was detected by Westadded, growth inhibition and increased apoptosis of MG453 cells were detected. The protein expression level of livin was down-regulated obviously (P 〈 0. 05 ). Conclusion PDTC can promote the apoptosis of MTX-treated MG-63 cells, which may be correlated with down-regulation of the livin expression.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第5期787-788,共2页
Chinese Journal of Experimental Surgery
基金
辽宁省教育厅科技研究项目(2008557)