虫草与富硒虫草多糖的体外抗氧化活性

Antioxidant Activity of Intracellular and Extracellular Polysaccharides from Regular and Se-rich Cordyceps sinensis in vitro

采用Fenton法和邻苯三酚自氧化法测定虫草和富硒虫草多糖对.OH、O2-.和H2O2的清除率。结果表明:虫草多糖对O-2.和H2O2的清除能力依次为富硒虫草胞外多糖>富硒虫草胞内多糖>虫草胞外多糖>虫草胞内多糖,而对.OH的清除能力依次为:富硒虫草胞外多糖>虫草胞外多糖>富硒虫草胞内多糖>虫草胞内多糖;当100mg/L多糖溶液添加量分别高于80、40、90μL时,各组多糖对.OH、O2-.和H2O2 3种自由基的抑制率均可高于50%。

The scavenging effects of polysaccharides from the mycelia（intracellular） and culture supernatant（extracellular） of Cordyceps sinensis after 6 days of cultivation were determined against hydroxyl free radicals generated in a Fenton reaction system,superoxide anion free radicals（by pyrogallol auto-oxidation method） and hydrogen peroxide.The extracellular polysaccharides from Se-rich Cordyceps sinensis had the strongest ability to scavenge superoxide anion free radicals and hydrogen peroxide,followed by the intracellular polysaccharides from Se-rich Cordyceps sinensis as well as the extracellular and the intracellular polysaccha-rides from common Cordyceps sinensis.By the scavenging effect against hydroxyl free radicals,the four polysaccharides could be ranked in the following order： the extracellular polysaccharides from Se-rich Cordyceps sinensis,the extracellular polysaccharides from common Cordyceps sinensis,the intracellular polysaccharides from Se-rich Cordyceps sinensis and the intracellular polysaccharides from common Cordyceps sinensis.Adding each of the four polysaccharide solutions（100 mg/L） at a level of no less than 80,40μL and 90μL had a scavenging rate of over 50% against hydroxyl and superoxide anion free radicals as well as hydrogen peroxide,respectively.