肉鸡中魏斯菌属细菌的分离与鉴定

Isolation and Identification of Weissella Strains in Chicken

应用16S DNA基因测序法对肉鸡中分离出的可疑魏斯菌属细菌菌株进行鉴定。从沈阳某养鸡场抽取病死鸡在无菌条件下采集样品10份,微需氧条件下进行细菌分离纯化,比对魏斯菌属的基本形态和革兰氏染色特点,得到与魏斯菌属类似菌株14株。采用16S rDNA基因测序方法对分离出的细菌进行基因序列分析。在NCBI网站用BLAST软件在GenBank上进行同源性比较,比对结果表明编号为X1001、X1002、X1006、X1008、X1009的株菌同源性达90%。生化实验结果表明,X1006号菌株为魏斯菌属中的融合魏斯菌属菌株。

The suspicious Weissella strains isolated from chicken were identified by 16S rDNA gene sequencing.Under sterile conditions,10 dead broiler samples were collected from a certain chicken farm in Shenyang.The bacteria in each sample were isolated and purified under micro-aerobic conditions.Totally,14 bacterial strains were obtained and identified as Weissella.PCR based 16S rDNA gene sequencing showed that X1001,X1002,X1006,X1008 and X1009 strains had high homology（above 90%）.The biochemical experimental results revealed that X1006 strain had the characteristics of Weissella confusa.