桑黄胞内多糖免疫及抗氧化活性研究

Immune Function and Antioxidant Activity of Intracellular Polysaccharides from Phellinus baumii

通过腹腔注射环磷酰胺建立免疫抑制小鼠动物模型,考察桑黄粗多糖(SH)、纯化多糖组分(P-47000、P-8700)对增强免疫及抗氧化水平的变化情况,明确不同纯度多糖组分生物活性间的差异。结果表明:S H、P-47000、P-8700对环磷酰胺诱导的免疫功能低下小鼠具有不同程度的免疫促进作用,其顺序为:P-8700>SH>P-47000;P-47000的还原能力低于SH和P-8700,SH和P-8700还原能力相差不大;P-47000几乎对超氧阴离子自由基(O-2.)没有清除效果,P-8700和SH的清除率比较低,且P-8700略大于SH;P-8700对羟自由基(.OH)具有较好的清除效果,清除率达到53.421%,且P-8700的清除率高于SH和P-47000,P-47000清除率最低;不同纯度多糖对DPPH自由基的清除效果均较高,SH达到93.221%;P-8700清除效果最低,为66.435%。

An immune-deficient mouse model was established by intraperitoneal injection of cyclophosphamide（CTX） to explore the immune enhancement and antioxidant capability of crude polysaccharides（SH） from Phellinus banmii and purified polysaccharide fractions（P-47000 and P-8700）.Meanwhile,the bioactivity differences among SH,P-47000 and P-8700 were investigated.The results showed that SH,P-47000,and P-8700 could enhance immune function of CTX-induced immune-deficient mice to some extent.The order of immune enhancement function from strong to weak was P-8700,SH and P-47000.P-47000 exhibited a lower reducing power than SH and P-8700.However,no significant difference in reducing power between SH and P-8700 was observed.In addition,P-47000 revealed lower ability to scavenge superoxide anion free radicals than SH and P-8700.Moreover,P-8700 revealed stronger hydroxyl free radical scavenging capacity with a scavenging rate of 53.421%,which was obviously higher than that of SH and P-47000.All the polysaccharides had high scavenging capability against DPPH free radicals.SH and P-8700 revealed the highest and lowest scavenging capability on DPPH free radicals with a scavenging rate of respectively 93.221% and 66.435%.