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四环素诱导调控表达系统的研究与应用 被引量：7

Research progress and application of Tet inducible regulated expression system
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摘要 四环素(Tet)诱导调控表达系统是在大肠杆菌Tn10转座子中特异的Tet抗性操纵子基础上建立起来的一种用于诱导基因表达的调控系统。经过近几年来的发展,衍生出了Tet-on、Tet-off两套调控系统;随着对Tet诱导调控表达系统的改进,其对基因表达调控的严谨性、诱导效率及安全性等方面逐步得到改善,并被广泛应用于基础研究及体内外的实验治疗研究。此外Tet诱导调控系统与其他诱导调控体系及新兴技术的联合应用,为疾病的基因治疗研究提供有力的手段。 Tet inducible regulated expression system used for inducing gene expression is established on the basis of specific tetracycline resistance operon present in transposon of E.coli Tn10.After years of development,two types of systems named Tet-on and Tet-off system have respectively derived from the original one.With the improvement of Tet inducible regulated expression system,the stringency,efficiency and safety have been gradually increased,which has been applied to basic researches and experimental therapeutic studies in vivo and in vitro.Tet inducible regulated expression system combined with other regulated systems and new developing technologies provides an efficient method for gene therapy.
作者 陈皓 夏海滨
机构地区 陕西师范大学生命科学学院基因治疗研究室
出处 《生命的化学》 CAS CSCD 北大核心 2011年第2期285-291,共7页 Chemistry of Life
基金 国家自然科学基金项目(30872993)资助
关键词 四环素 基因治疗 诱导调控 tetracycline gene therapy inducible regulation
分类号 Q78 [生物学—分子生物学]
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参考文献39

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二级参考文献13

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共引文献2

同被引文献47

  • 1王勇,王峰超,魏泓,倪勇,吴军,高翔.皮肤组织特异性表达hCTLA4-Ig转基因小鼠品系的建立[J].Acta Genetica Sinica,2005,32(9):916-922. 被引量：3
  • 2Zhou Zhu,Tao Zheng,Chun G Lee,Robert J Homer,Jack A Elias.Tetracycline-controlled transcriptional regulation systems: advances and application in transgenic animal modeling[J]Seminars in Cell and Developmental Biology,2002(2).
  • 3Stéphane Y Corbel,Fabio M.V Rossi.Latest developments and in vivo use of the Tet system: ex vivo and in vivo delivery of tetracycline-regulated genes[J]Current Opinion in Biotechnology,2002(5).
  • 4Kappel S,Matthess Y,Kaufmann M, et al.Silencing of mammalian genes by tetracycline-inducible shRNA expressionNature Protocols,2007.
  • 5Wang QZ,Lv YH,Diao Y,et al.The design of vectors for RNAidelivery systemCurrent Pharmaceutical Design,2008.
  • 6Cheng TL,Chang WT.Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cellsMethods in Molecular Biology,2007.
  • 7Weber W,Fussenegger M.Pharmacologic transgene control systems for gene therapyThe Journal of Genetic Medicine,2006.
  • 8Berens C,Hillen W.Gene regulation by tetracyclines:Constraints of resistance regulation in bacteria shape TetRfor application in eukaryotesEuropean Journal of Biochemistry,2003.
  • 9ZHANG H,TAKAYAMA K,ZHANG L,et al.Tetracycline-inducible promoter-based conditionally replicative adenoviruses for the control of viral replicationCancer Gene Therapy,2009.
  • 10ROGNONI E,WIDMAIER M,HACZEK C,et al.Adenovirus-based virotherapy enabled by cellular YB-1 expression in vitro and in vivoCancer Gene Therapy,2009.

引证文献7

二级引证文献12

生命的化学
2011年 第2期

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