人参多糖对K562细胞基因表达谱的影响

Effect of ginseng polysaccharide on gene expression profile of K562 cells

目的利用基因表达谱芯片研究人参多糖对体外培养的人红白血病K562细胞基因表达谱的影响。方法人参多糖处理K562细胞48 h后,分别提取给药组和对照组K562细胞总RNA,将两组RNA纯化为mRNA,逆转录成cDNA,用2种不同的荧光染料Cy3和Cy5进行线性扩增标记,与Illumina全基因组表达谱基因芯片杂交,采用生物信息学方法分析人参多糖处理后K562细胞基因表达谱的改变。结果共发现差异基因306个,其中上调基因220个,下调基因86个,并对其进行生物学功能分类。结论诱导肿瘤细胞分化是多基因作用的综合结果,筛选的基因对研究肿瘤发生、发展与逆转分化,以及寻找潜在的抗肿瘤药物作用靶点可能有意义。

Objective To investigate the effect of ginseng polysaccharide on the gene expression profile of human erythroleukemia K562 cells cultured in vitro.Methods The total RNAs treated with ginseng polysaccharide for 48 h were extracted from both K562 cells and the control K562 cells.All the RNAs were purified into mRNA and then convened to double-stranded cDNAs by reverse transcription.The cDNAs were labeled with a fluorescence dye Cy3 or Cy5 and hybridized to a Illumina whole-genome oligonucleotide microarray.The change in the gene expression profile of K562 cells following treatment with ginseng polysaccharide was analyzed by using bioinformatics.Results A total of 306 differential genes were found.Of which 220 were up-regulated and 86 were down-regulated.Their biologic function was classified.Conclusion Tumor cell differentiation is multiple genes comprehensive results,the screening of the genes may be meaningful for understanding tumorigenesis,tumor development,reverse polarization,and finding potential drug targets.