枯草芽孢杆菌对肉鸡生长性能和肠黏膜抗氧化及免疫功能的影响

Effects of Bacillus Subtilis on Growth Performance,Antioxidant Capacity and Immunity of Intestinal Mucosa in Broilers

试验旨在研究肉鸡日粮中添加枯草芽孢杆菌对其生长性能及其肠黏膜抗氧化和免疫功能的影响。将600羽1日龄健康振宁雏公鸡随机分成4组,每组设3个重复,每个重复50羽。处理组Ⅰ为对照组,饲喂基础日粮;处理组Ⅱ、Ⅲ、Ⅳ分别在基础日粮中添加0.25、0.50、0.75 g/kg的枯草芽孢杆菌制剂(活菌数为108 CFU/g)。试验期77 d。结果表明:与对照组相比,处理组Ⅱ和Ⅲ组肉鸡的日增重有所提高但无显著差异,处理组Ⅳ显著提高了6.86%(P<0.05);空肠黏膜中总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、维生素C和黄嘌呤氧化酶(XOD)含量显著高于对照组(P<0.05),丙二醛(MDA)明显降低(P<0.05);盲肠和回肠黏膜中细胞因子IL-2、IL-4、IL-10和TNF-α含量均比对照组极显著提高(P<0.01)。提示肉鸡日粮中,添加适量的枯草芽孢杆菌制剂能改善其肠黏膜的抗氧化和免疫功能,从而提高肉鸡的生长性能。

This experiment was conducted to study the effect of Bacillus subtilis on growth performance,antioxidant capacity and immunity of intestinal mucosa in broilers.Six hundred one-day cocks were randomly assigned to four treatments with three replicates and fifty cocks with each pen.The groups received the same basal diet supplemented with 0,0.25,0.50,0.75 g/kg praeparatum of Bacillus subtilis,respectively.The feeding trial lasted 11 weeks.The results showed that compared to the control,the average daily gain in 0.75 g/kg group increased significantly by 6.86%（P0.05）;The contents of T-AOC,GSH-Px,VC and XOD in jejunal mucosa of test group were significantly higher（P0.05） than that of control group,while the content of MDA was significantly decreased（P0.05）.The contents of IL-2,IL-4,IL-10 and TNF-α in appendix mucosa and ileal mucosa of test group were significantly increased（P0.01）.These results indicated that adding Bacillus subtilis in diet could improve antioxidant capacity and immunity of intestinal mucosa,which could improve broilers＇ growth performance.