柱前衍生化法测定盐酸氨基葡萄糖软膏的含量

Determination of the content of glucosamine hydrochloride in glucosamine hydrochloride ointment by pre-column derivatization RP-HPLC

目的建立柱前衍生化RP-HPLC法测定盐酸氨基葡萄糖软膏的含量。方法采用NH2色谱柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水（体积比90∶10）,流速为1.0 mL.min-1,衍生化试剂为异硫氰酸苯酯,检测波长254 nm。结果在本色谱条件下,盐酸氨基葡萄糖质量浓度在12.75~38.25 mg.L-1内呈良好的线性关系,回收率为100.4%。结论该方法准确、可靠,适用于盐酸氨基葡萄糖软膏的含量测定。

Objective To establish a pre-column derivatization RP-HPLC method to determine the glucosamine hydrochloric in glucosamine hydrochloric ointment.Methods RP-HPLC was adopted with an NH2 column（250 mm× 4.6 mm,5 μm）,a mobile phase consisting of acetonitrile-water（V∶V=90∶10）,at 1.0 mL·min-1.The UV detective wavelength was set at 254 nm.Results Glucosamine hydrochloric was well separated.The method showed a good linearity for glucosamine hydrochloric in range of 12.75-38.25 mg·L-1（r=0.999 3）.The average recovery was 100.4%.Conclusions The method can be used to determine glucosamine hydrochloric in glucosamine hydrochloric ointment.