摘要
目的建立柱前衍生化RP-HPLC法测定盐酸氨基葡萄糖软膏的含量。方法采用NH2色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(体积比90∶10),流速为1.0 mL.min-1,衍生化试剂为异硫氰酸苯酯,检测波长254 nm。结果在本色谱条件下,盐酸氨基葡萄糖质量浓度在12.75~38.25 mg.L-1内呈良好的线性关系,回收率为100.4%。结论该方法准确、可靠,适用于盐酸氨基葡萄糖软膏的含量测定。
Objective To establish a pre-column derivatization RP-HPLC method to determine the glucosamine hydrochloric in glucosamine hydrochloric ointment.Methods RP-HPLC was adopted with an NH2 column(250 mm× 4.6 mm,5 μm),a mobile phase consisting of acetonitrile-water(V∶V=90∶10),at 1.0 mL·min-1.The UV detective wavelength was set at 254 nm.Results Glucosamine hydrochloric was well separated.The method showed a good linearity for glucosamine hydrochloric in range of 12.75-38.25 mg·L-1(r=0.999 3).The average recovery was 100.4%.Conclusions The method can be used to determine glucosamine hydrochloric in glucosamine hydrochloric ointment.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2011年第5期380-383,共4页
Journal of Shenyang Pharmaceutical University