镰刀菌酸及其枯萎病菌培养液对棉花的毒性

Virulence of Fusaric Acid and Rude Extract of Fusarium oxysporum on Cotton

[目的]了解镰刀菌酸及其枯萎病菌培养滤液对棉花感病品种及其抗性转化品系的致萎作用。[方法]用2种类型毒素制品浸泡棉花种子及幼苗,以无菌水作为CK,根据浸泡的时间不同测定种子萌发率及病情指数。[结果]25%培养滤液中镰刀菌酸的浓度为21.46μg/ml,但其处理种子萌发率却比30.00μg/ml的纯镰刀菌酸处理低,50%培养滤液镰刀菌酸的浓度为30.62μg/ml,其处理种子萌发率与50.00μg/ml的纯镰刀菌酸处理一样。病原菌培养滤液浓度不同、处理后时间不同,其病情指数均不同,随着处理后时间的延长和处理浓度的增加,病情指数也大都呈逐渐增加趋势。[结论]病原菌培养滤液对种子萌发的抑制作用要高于纯镰刀菌酸。抗性转化品系与原感病品系相比对病原菌培养滤液的抗性明显增强。

[Objective] The aim was to study wilt-produced effect of fusaric acid and culture liquid on cotton diseased varieties and resistant conversion strains.[Method] Cotton seeds and seedlings of different resistance varieties were incubated within FA and rude extract of the different concentrations and time.The virulence of two toxins was evaluated adding to germination rate of seeds and incidence of seedlings,respectively.[Result] In 25% culture liquid,the concentration of fusaric acid is 21.46 μg/ml,its inhibition rate of seed germination is lower than 30.00 μg/ml.In 50% culture liquid,the concentration of fusaric acid is 30.62 μg/ml,its inhibition rate of seed germination is the same with 50.00 μg/ml.With the extending of incubating time and dose of extract,the disease index of cotton seedlings increase.[Conclusion] The virulence of rude extract of Fusarium oxysporum is stronger than fusaric acid on cotton seeds and seedlings.