北陆花青素的抗氧化作用

Antioxidative Activity of Anthocyanins from Northland

[目的]探讨北陆花青素的抗氧化作用。[方法]分别采用Fenton反应体系、邻苯三酚自氧化体系、亚油酸体系和普鲁士蓝法来测定北陆花青素对OH.和O2-.的清除作用、对脂质过氧化物的抑制作用及其还原能力进行评价,并以维生素C和葡萄籽花青素作为阳性对照。[结果]北陆花青素的抗氧化能力高于葡萄籽花青素和维生素C,对OH.和O2-.具有很强的清除作用,其IC50分别为0.345mg/ml和34.28μg/ml,对脂质过氧化物有明显的抑制作用并有较高的还原能力。[结论]北陆花青素是一种潜在的具有抗氧化活性的天然药物。

[Objective] To explore antioxidative activity of anthocyanins from Northland.[Method] Using anthocyanins from grape seeds and vitamin C as positive control,Fenton reacting system,pyrogallol autoxidation system,linoleic acid system and Prussian blue method were respectively employed to determine OH· scavenging activity,O2-·scavenging activity,inhibitive effect on lipid peroxidation and reducing capacity of anthocyanins from Northland.[Result] Antioxidative activity of anthocyanins from Northland was higher than that in anthocyanins from grape seeds and vitamin C.Anthocyanins from Northland had strong scavenging activities on OH· and O2-·,IC50 of which was 0.345 mg/ml and 34.28 μg/ml,respectively.Furthermore,it was also provided with obvious inhibiting activity on lipid peroxidation and reducing capacity.[Conclusion] Anthocyanins from Northland may be a potential natural medicine with antioxidative activity.