双浊点萃取-毛细管电泳法分离测定井水中痕量硝基苯酚位置异构体

Determination and Isolation of the Nitrophenol Isomers in Well Water with Dual-Cloud Point Extraction and Capillary Electrophoresis

[目的]为测定井水中痕量硝基苯酚位置异构体提供参考。[方法]采用双浊点萃取进行预处理,结合毛细管电泳技术对萃取物进行检测。[结果]选择浓度为0.5%Triton X-114为表面活性,pH 1.5,添加浓度0.020 g/ml NaCl,同时添加浓度0.2 mol/L NaOH溶液100μl,40℃时加热20min条件下双浊点萃取-毛细管电泳法分离测定井水中痕量硝基苯酚位置异构体,邻硝基苯酚、间硝基苯酚在0.000 2～0.005 0 mg/ml浓度范围内均呈现良好的线性关系,方法的加标回收率可达97%,相对标准偏差均小于5%。[结论]该方法消除了富集相中表面活性剂对毛细管电泳分离的影响,分析结果准确,且对环境友好。

[Objective] The reference for the determinate of the nitrophenol isomers in well water provided through the research.[Method] The material from well water was pretreatment with Dual-Cloud Point Extraction and measured with Capillary Electrophoresis.[Results] In the optimum condition,which concentration of the surfactant Triton X-114 was 0.5%,the pH was 1.5,the adding amount of NaCl was 0.020 g/ml,meanwhile,the adding amount of 0.2 mol/L NaOH was 100 μl,the nitrophenol isomers in well water were tested with the method of Dual-Cloud Point Extraction and Capillary Electrophoresis at the temperature of 40 ℃ for 20 minutes.There was good linearity between O-nitrophenol and M-nitrophenol in the range of 0.000 2-0.005 0 mg/mL,the recovery of the technique reached 97% and the relative standard deviation was less than 5%.[Conclusion] The result from the technique was accurate and environment-friendship because the effect of the surfactant in the accumulation phase on the isolation of capillary electrophoresis was eliminated.