WT1反义寡核苷酸对人卵巢癌裸鼠皮下移植瘤形成的抑制作用

Inhibitory Effect of Antisense Oligodeoxynucleotide Targeting WTl on the Human Ovarian Carcinoma Transplanted Subcutaneously in Nude Mice

目的:观察WT1反义寡核苷酸作用后的人卵巢癌细胞株SKOV3在裸鼠皮下的成瘤能力。方法:应用反义寡核苷酸(ASODN)技术,将WT1反义寡核苷酸转染到SKOV3细胞中,然后将这种被转染的细胞接种于裸鼠皮下,观察肿瘤体积的变化,并计算抑瘤率。结果:脂质体介导的WT1反义寡核苷酸作用后的卵巢上皮癌细胞在裸鼠皮下成瘤能力降低,首次出现目检可见肿瘤的平均时间延长,抑瘤率明显增加。结论:WT1反义寡核苷酸能降低卵巢癌SKOV3细胞的成瘤能力,抑制肿瘤生长,在上皮性卵巢癌的基因治疗中有一定价值。

Objective： To study the inhibitory effect of WT1 antisense oligodeoxynucleotides （ASODN） transfection on the human ovarian epithelial cancer transplanted subcutaneously in nude mice. Methods： Ovarian carcinoma cell SKOV3 lines were treated with WT1-ASODN, and then transplanted subcutaneously in nude mice. The changes of tumor volume were observed and the tumor growth inhibitory rate was calculated. Results： The tumorigenic ability of ovarian carcinoma cell lines SKOV3 transfected by WT1 ASODN was reduced, the time that tumor could be first detected was prolonged and the maximum tumor growth inhibitory rate was increased. Conclusion： WT1 antisense phosphorothioate oligonucleotides can inhibit the tumorigenesis of ovarian epithelial carcinoma cell lines in nude mice. It maybe a valuable gene therapy for the ovarian epithelial carcinoma.