夏枯草化学成分的研究

STUDIES ON THE CHEMICAL CONSTITUENTS FROM PRUNELLA VULGARIS

目的：研究夏枯草（ Ｐｒｕｎｅｌｌａ ｖｕｌｇａｒｉｓ Ｌ．） 的化学成分。方法：用现代各种色谱方法分离夏枯草化学成分，用波谱技术鉴定结构。结果：从夏枯草的干燥果穗中分得６ 个化合物，经波谱（ Ｉ Ｒ， Ｕ Ｖ， Ｆ Ａ Ｂ Ｍ Ｓ，１ Ｄａｎｄ ２ Ｄ Ｎ Ｍ Ｒ）解析分别鉴定为３β，１６α，２４三羟基齐墩果１２烯２８酸３ Ｏ（６′丁酰基）β Ｄ吡喃葡糖苷（ Ｉ） ，乌苏酸（ Ｉ Ｉ） ，２α，３α二羟基乌苏１２烯２８酸（ Ｉ Ｉ Ｉ） ，槲皮素（ Ｉ Ｖ） ，槲皮素３ Ｏβ Ｄ半乳糖苷（ Ｖ） 和咖啡酸乙酯（ Ｖ Ｉ） 。结论： Ｉ为新化合物，命名为夏枯草皂苷 Ｂ（ｖｕｌｇａｒｓａｐｏｎｉｎ Ｂ） ， Ｉ Ｉ Ｉ为首次从该植物中分离得到， Ｖ， Ｖ Ｉ为首次从该属植物中分离得到。

AIM: To find the chemical constituents of Prunella vulgaris L. METHODS: To separate the constituents of Prunella vulgaris by using various kinds of chromatography and identify their structures on the basis of spectral analysis. RESULTS: Six compounds were isolated from the spikes of Prunella vulgaris L. Their structures were established as 3β,16α,24 trihydroxyoleana 12 en 28 oic acid 3 O (6′ butyryl) β D glucopyranosid (I), ursolic acid (II), 2α,3α dihydroxyurs 12 en 28 oic acid (III), quercetin (IV), quercetin 3 O β D galactoside (V) and ethyl caffeate (VI) based on the analysis of spectral data of IR, UV, FAB MS, 1D and 2D NMR. CONCLUSION: I was a new compound, named vulgarsaponin B, III was isolated for the first time from this plant, V and VI for the first time from the Prunella genus.