超临界流体萃取-高效液相色谱法测定何首乌中磷脂成分

DETERMINATION OF PHOSPHOLIPID IN POLYGONUM MULTIFLORUM THUNB. BY SUPERCRITICAL FLUID EXTRACTION — HPLC

目的：建立超临界流体萃取（ Ｓ Ｆ Ｅ） 中药何首乌中的磷脂类成分的方法。方法：采用系统观察法考察了 Ｓ Ｆ Ｅ 的提取工艺，并用反相高效液相色谱法进行分离测定。结果：在 Ｓ Ｆ Ｅ 中，通过对萃取条件的考察，确定萃取压力为３１５ Ｍ Ｐａ ，温度５０ ℃，改性剂加入量０６ ｍ Ｌ·ｇ － １ ，静态萃取时间５ ｍｉｎ 及动态萃取体积７ ｍ Ｌ。在 Ｈ Ｐ Ｌ Ｃ 中，以 Ｗａｔｅｒｓ Ｓｙｍ ｍｅｔｒｙ Ｃ１ ８ 为固定相，甲醇—１０ ％ 磷酸溶液（９０∶１０） 为流动相，检测波长２０６ ｎ ｍ 。结论： Ｓ Ｆ Ｅ Ｈ Ｐ Ｌ Ｃ 法测定何首乌中的磷脂类成分，为何首乌及其制剂的质量控制提供依据。

AIM: To develop a supercritical fluid extraction (SFE) method for the phospholipids of Polygonum multiflorum Thunb. METHODS: SFE was selected by systemic research method, an HPLC method was developed for the determination of phosphatidylethanolamine and phosphatidylcholine. RESULTS: SFE conditions: pressure 31 5 MPa, temperature 50℃, 0 3 mL·g -1 modifier. The samples were extracted statically for 5 min, and subsequently extracted dynamically with 7 mL extractant. HPLC conditions: The HPLC column was packed with C 18 (5 μm). The mobile phase was methanol—1% phosphoric acid (90∶10). The detection wavelength was 206 nm. CONCLUSION: The method is accurate, convenient and rapid, and can be a measure of quality control for Polygonum multiflorum Thunb. and its preparations.