生防细菌1505的鉴定及其对采后柑橘炭疽病的抑制效果

Identification of Bio-Control Strain 1505 and Its Effect on Controlling Postharvest Citrus Anthracnose

采用细菌学方法及分子生物学技术对菌株1505进行鉴定,并对其在防治采后柑橘炭疽病上的效果进行研究。结果显示,菌株1505对柑橘炭疽病菌具有拮抗活性,其活菌悬浮液在PDA平板上对柑橘炭疽病菌产生明显抑制作用,而灭菌发酵液和去除菌体的发酵液均无抑菌效果;连续8次在人工培养基上转代培养,菌株1505对柑橘炭疽病菌生长的抑制力没有发生明显改变;菌株1505对胶孢炭疽菌[Colletotrichum gloeosporioides(Penz.)Sacc.]引起的柑橘炭疽病有明显的防治作用,刺伤接种的防效为48.8%,自然发病的防效为46.3%。根据形态学特征和生理生化反应,结合16S rDNA序列分析结果,将菌株1505鉴定为枯草芽孢杆菌(Bacillus subtilis)。

The present study aimed to use the bacteriological method and molecular biology technique to identify the strain 1505 and study its efficacy in controlling postharvest Citrus anthracnose.The results showed that the strain 1505 had antagonistic activity against Colletotrichum gloeosporioides.Its cell suspension showed significant suppressive activity against C.gloeosporioides,while no suppressive activity was detected in high-temperature-sterilized fermentation fluid or cell-free fermentation fluid.Less change in the suppressive ability of strain 1505 against C.gloeosporioides was observed during 8 continuously subculture in artificial media.The strain 1505 had significant efficacy in controlling Citrus anthracnose disease causing by C.gloeosporioides.The average efficacies of strain 1505 in controlling the disease were 48.8 % in artificial inoculation test and 46.3 % in natural infection test.Strain 1505 was identified as Bacillus subtilis based on the physiological and biochemical properties and its 16S rDNA sequence analysis.