双核丝核菌融合群AG-R的分离鉴定

Isolation and Identification Research of Binucleate Rhizoctonia SPP. AG-R

从采自成都、德宏、广西芋头球根上分离到丝核菌,经融合反应、ITS序列分析鉴定得出,该分离菌株属于双核丝核菌中的AG-R群。分离菌株对白菜幼苗有较强的致病力。从芋头球根中能分离到丝核菌,分离频率很高,从芋头球根外观上看,丝核菌对它没造成危害,植株生长正常。国内尚未见从芋头球根中能分离到丝核菌AG-R的研究报道。

Rhizoctonia spp.was obtained from taro＇s bulbs in Chengdu,Dehong and Guangxi.According to anastomosis,sequence analysis of 5.8s rDNA-ITS of isolates,these isolates were categorized as AG-R.In pathogenicity test on the seedlings of Chinese cabbage,isolates showed high virulence on seedlings.Rhizoctonia spp.could be isolated from taro with high frequency,and Rhizoctonia spp.did not cause disease to outside taro and plants could grow normally.