山豆根多糖对小鼠胸腺和脾脏淋巴细胞凋亡的影响

Influence of Sophora subporsrate Polysaccharide on Apoptosis of Thymocyte and Spleen Lymphocytes in Mouse

为探讨山豆根多糖对地塞米松诱导的小鼠胸腺和脾脏淋巴细胞凋亡的影响,通过腹腔注射地塞米松建立小鼠胸腺和脾脏淋巴细胞凋亡模型,并采用琼脂糖凝胶电泳、透射电子显微镜和流式细胞术进行观察和检测。结果表明,除空白对照组外,其余各组的琼脂糖凝胶电泳图均出现梯状条带,而且透射电子显微镜观察凋亡对照组和不同剂量山豆根多糖组均可见典型的凋亡特征,胸腺和脾脏淋巴细胞的胞核染色质凝聚呈半月形、C形及凋亡小体,可见巨噬细胞吞噬凋亡小体。流式细胞仪分析显示,不同剂量山豆根多糖组凋亡率与凋亡对照组凋亡率比较有显著性差异(P<0.01),且随着山豆根多糖剂量增大,凋亡率降低,山豆根多糖腹腔注射剂量为400 mg/kg时凋亡率最低,表明山豆根多糖剂量为50～400 mg/kg时对地塞米松诱导的小鼠胸腺和脾脏淋巴细胞凋亡有明显的抑制作用。

The study was carried out to investigate the effects of Sophora subporsrate polysaccharide on the apoptosis of thymocyte and spleen lymphocytes in mouse induced by dexamethasone,and it was investigated and detected by methods of agarose gel electrophoresis,transmission electron microscopy,flow cytometry,apoptosis mode of hymocyte and spleen lymphocytes was constructed by intraperitoneal injection on mouse.DNA agarose electrophoresis showed that the apoptosis control group and Sophora subporsrate polysaccharide groups with different doses emerged a typical ladder.The characteristics of nucleus chromatin of thymocyte and spleen lymphocytes showed the shape of a half moon,＇C＇ and apoptotic bodies and peritoneal macrophage phagocytizing apoptotic bodies has been found under transmission electron microscopy.Flow cytometry analysis showed that apoptosis rate in group of Sophora subporsrate polysaccharide group with different doses decreased significantly compared with the apoptosis control group（P0.01）,and with the increasing dose of Sophora subporsrate polysaccharide,the apoptosis ratio was decreased.The lowest rate of apoptosis was observed at the dose of 400 mg/kg Sophora subporsrate polysaccharide.In conclusion,Sophora subporsrate polysaccharide at 50-400 mg/kg dose had significant inhibitory effects on the apoptosis of thymocyte and spleen lymphocytes in mouse induced by dexamethasone.